特发性血小板减少性紫癜患者T细胞及B细胞亚群变化的研究  

T and B lymphocyte subsets in idiopathic thrombocytopenic purpura patients

作　　者：吕艳 刘文文 王聪 武有聪 白丽 Lyu Y Liu WW Wang C Wu YC Bai Li Bai L(Comprehensive Laboratory Pathogen of Basic Medical College, Dali University, Dali 671000, China Yunnan Key Laboratory for Biomedical Research and Development of lnsects ,Dalir University,Dali 671000, China)

机构地区：[1]大理大学基础医学院病原综合实验室,671000 [2]大理大学云南省昆虫生物医药研发重点实验室

出　　处：《国际免疫学杂志》2017年第1期21-25,共5页International Journal of Immunology

基　　金：基金项目：国家自然科学基金（30860262）;云南省教育厅科学研究基金研究生项目（2013J112）

摘　　要：目的探讨特发性血小板减少性紫癜（idiopathicthrombocytopenicpurpura，ITP）患者T、B淋巴细胞亚群的变化。方法从大理州人民医院收集ITP患者和健康者的外周血，用Sysmex血液分析仪及流式细胞术分析T细胞及B细胞亚群。结果与健康人群比较，ITP患者CD3＋T细胞百分率无明显变化，CD4＋T细胞百分率降低（42．39％±12．12％，P〈0．05），CD8＋T细胞百分率升高（46．93％±11．99％，P〈0.05）；CD19＋B细胞百分率升高（14．11％±10．28％，P〈0．05），T2B细胞百分率（34．51％±9．70％,P〈0．05）、成熟B细胞百分率（30．91％±7．12％，P〈0．05）及记忆性B细胞百分率（23．31％±8．23％，P〈0．05）增多，差异均有统计学意义。ITP患者T1B细胞、Kappa＋B细胞、Lambda＋B细胞百分率与健康人差异无统计学意义。结论ITP患者外周血T细胞亚群和B细胞亚群分布发生了变化，这种变化在ITP发病中的作用尚需进一步研究。Objective To investigate the changes of T cell and B cell subsets in idiopathic thrombocyto- penic purpura（ITP） patients. Methods T cells and B cells and their subsets in the peripheral blood from ITP patients and healthy volunteers in a hospital of Dali were analyzed with Sysmex hematology analyzers and flow eytometry. Results Compared with the healthy population, the results show that the percentage of CD3 ＋ T cells had no significant change and CD4＋ T cells reduced （42.39%± 12. 12% ,P 〈0.05） ,but CD8 ＋ T cells in- creased（46.93% ±11.99% ,P 〈0.05）. CD19＋ B cells （14. 11% ±10.28%,P 〈0.05）,T2 B cells （34. 51% ＋9.70% ,P 〈0.05） ,mature B cells （30.91% ±7.12% ,P 〈0.05） and memory B cells increased （23.31% ± 8.23%, P 〈 0.05 ） in ITP patients. The differences were statistically significant. T1 B cells, Kap- pa ＋ B cells, Lambda ＋ B cells show no significant change in ITP patients. Conclusion Preliminary studies have shown that some T cell subsets and B cell subsets of ITP patients were changed, and the role of these changes in ITP requires further study.

关 键 词：特发性血小板减少性紫癜 T淋巴细胞亚群 B淋巴细胞亚群 

分 类 号：R446.6[医药卫生—诊断学] R554.6[医药卫生—临床医学]