赤霉素与小麦生理型雄性不育的关系  

Relationship between Gibberellin and Chemical-Induced Male Sterility in Wheat

作　　者：巴青松[1] 张改生[2] 李桂萍[1] 张根生[1] 周丽娟[1] 宋运贤[1] 陈楚[1] 傅兆麟[1] BA Qingsong ZHANG Gaisheng LI Guiping ZHANG Gensheng ZHOU Lijuan SONG Yunxian CHEN Chu FU Zhaolin(Key Laboratory of Plant Resources and Biology/College of Life Science, Huaibei Normal University Huaibei,Anhui 235000,China Key Laboratory of Crop Heterosis of Shaanxi Province/ College of Agronomy, Northwest A＆F F University, Yangling, Shaanxi 712100, China)

机构地区：[1]淮北师范大学生命科学学院/资源植物生物学安徽省重点实验室,安徽淮北235000 [2]西北农林科技大学农学院/陕西省作物杂种优势研究与利用重点实验室,陕西杨凌712100

出　　处：《麦类作物学报》2017年第3期344-348,共5页Journal of Triticeae Crops

基　　金：安徽省自然科学基金项目(1708085QC62);安徽省高等学校省级自然科学研究项目(KJ2014B19;KJ2016A624;KJ2013A232);淮北师范大学博士科研启动基金项目(15600971;12601033)

摘　　要：为探究赤霉素与小麦生理型雄性不育的关系,以新型小麦化学杀雄剂SQ-1作为诱导剂,西农1376为材料,构建了西农1376不育和可育生理系,通过扫描电镜、荧光定量PCR、气相质谱等技术,研究了小孢子花粉形态、TaGAMYB和CYP709C1基因表达、硬脂酸和赤霉素含量和花粉育性。结果表明,化学杀雄剂SQ-1诱导的生理型雄性不育系花粉粒畸形,TaGAMYB和CYP709C1基因表达量显著下降,硬脂酸含量显著上升,赤霉素含量显著低于可育系。赤霉素对雄性不育系的育性有一定逆转效应。The study was conducted in order to reveal the relationship between gibberellin and chemical- induced male sterility in wheat（Triticurn aestivum L）. Wheat variety Xinong 1376 was explored to de- velop physiological male sterile line through spraying SQ-I（a new chemical agent） on wheat plants at Feeke＇s 8.5 stage,with the plants sprayed with same volume water as the control. Pollen morpholo gy, TaGAMYB and CYP709C1 expression, octadecanoic acid content, and gibberellin content were ana- lyzed via techniques such as scanning electron microscope, fluorescence quantitative PCR,gas chroma- tography mass spectrum at mononuclear stage. The results indicated that pollen deformity of chemi- cal-induced male sterility generated,and expression patterns of TaGAMYB and CYP709C1 were down- regulated significantly,and instead,octadecanoic acid content rise sharply. Meanwhile,gibberellin con- tent was significantly lower than that of fertile line. Gibberellin have a reverse effect on sterility of male sterile lines.

关 键 词：小麦 生理型雄性不育 赤霉素 花粉壁 

分 类 号：S512.1[农业科学—作物学] S330