药用爬山虎对CIA大鼠血清IL-17和关节滑膜细胞NF-κB表达的影响  

Influence of Medicinal Parthenocissus on the Expression of Serum levels IL-17 and Synovial Cells NF-κB in Collagen-Induced Rats with Rheumatoid Arthritis

作　　者：徐晖[1] 马武开[1] 唐芳[1] 宁乔怡 姚血明[1] 黄颖[1] 龙小琴[1] XU Hui MA Wu - kai TANG Fang NING Qiao - yi YAO Xue - ming HUANG Ying Long Xiao - qing(Department of Rheumatology and Immunology, The Second Affiliated Hospital of GuiYang University of CM, Guiyang 550001, Guizhou China)

机构地区：[1]贵阳中医学院第二附属医院风湿免疫科,贵州贵阳550001

出　　处：《贵阳中医学院学报》2017年第2期11-15,共5页Journal of Guiyang University of Chinese Medicine

基　　金：贵州省科技创新人才团队项目;项目编号:黔科合人才团队(2013)4033;贵阳中医学院院内课题;项目编号:[2012]09号;贵州省中医药重点学科项目(2012);国家卫生计生委中医临床重点专科项目(2012);贵州省中医药管理局课题;项目编号:QZYY2013-63

摘　　要：目的:探讨药用爬山虎对牛II型胶原诱导性关节炎大鼠(CIA)关节滑膜细胞核周因子-κB(NF-κB)表达及血清IL-17水平的影响。方法:将40只6周龄雌性wistar大鼠随机分为空白对照组、模型对照组、雷公藤多苷对照组和爬山虎治疗组各10只。除空白对照组外,其余各组建立CIA模型,造模第7天开始予爬山虎汤剂和雷公藤水溶液灌胃治疗,连续用药4周,空白对照组和模型对照组灌服等量生理盐水,于末次给药2h后采集血清,处死大鼠,摘取双后肢膝关节分离滑膜,采用ELISA检测血清IL-17的表达,Western-blot检测关节滑膜NF-κB/P65的蛋白表达。结果:与空白对照组比较,模型组大鼠血清IL-17表达增加,爬山虎和雷公藤能够明显降低IL-17的表达(P<0.05);模型对照组大鼠关节滑膜细胞NF-κB/P65蛋白水平明显高于空白对照组(P<0.05),爬山虎与雷公藤多苷治疗组均能降低CIA大鼠关节滑膜细胞NF-κB/P65的表达,两组无显著性差异(P>0.05)。结论:药用爬山虎能够降低CIA模型大鼠血清IL-17的表达,下调关节滑膜细胞NF-κB的蛋白水平。Objective：To study the influence of medicinal parthenocissus on the expression of serum levels IL- 17 and synovial cells NF-κB in collagen -induced rats with rheumatoid arthritis. Method： 40 6 -week -old female Wistar rats randomly divided into a blank group, a model control group, a tripterygium glucosides treatment group and a medicinal parthenocissus treatment group. CIA models were established in all groups except the blank control group. After 7 days of modeling, lavage with Parthenocissus Decoction and ripterygium glucosides were given for four weeks successively, while the blank control group and the model control group were administered the same amount of normal saline. The serum was collected after 2 hours of the last gavage treatment. Cut the knee joints of both back limbs and removed their synovial membrane after the rats were killed. ELISA was used to measure the expression levels of IL - 17, and Western - blot was used to detect the expression of synovial NF - kappa B/P65 protein. Results： Compared with the blank group, the expression of IL -17 in serum increased in the model group, but decreased significantly in the parthenocissus group and the tripterygium glucosides （P 〈 0.05）. In the model control group, cells were significantly higher than those in the control group （ P the levels of NF - κB/P65 protein in synovial 〈 0.05 ）. The expression of CIA in rat synovial cells of NF -κB/P65 decreased in the parthenocissus group and the tripterygium glucosides group. There was no significant differences between the two groups （P 〉 0. 05 ）. Conclusion： Medicinal parthenocissus could reduce the expression of IL - 17 in serum of CIA model rats, and down regulate the protein level in synovial cells of NF - κB.

关 键 词：类风湿关节炎 胶原诱导性关节炎 动物模型 NF-ΚB IL-17 爬山虎 

分 类 号：R285.5[医药卫生—中药学]