基于免疫组化法分析肺腺癌S100A6蛋白表达与临床特征的关系  

Analysis of S100A6 protein expression and the clinical significance of patients with lung adenocarcinoma based on the method of immunohistochemisty

作　　者：南岩东[1] 房延凤[1] 姜华[1] 金发光[1] 杨拴盈[2] 

机构地区：[1]第四军医大学唐都医院呼吸内科,西安710038 [2]西安交通大学第二附属医院呼吸内科,西安710004

出　　处：《中华肺部疾病杂志（电子版）》2017年第1期15-19,共5页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：国家自然科学基金资助项目(81001040);第四军医大学优秀文职人员基金(2011-01);第四军医大学唐都医院后备人才基金(5033)

摘　　要：目的探讨S100A6蛋白在肺腺癌中的表达及其临床意义。方法收集2007年1月至2009年1月西安交通大学第二附属医院、第四军医大学唐都医院手术切除的肺癌标本,共计98例肺腺癌及其癌旁正常肺组织,用免疫组织化学法检标本中S100A6蛋白的表达情况,分析S100A6蛋白表达与患者临床病理特征及生存预后的关系。结果 S100A6蛋白在肺腺癌组织中的表达显著高于正常肺组织(P<0.001);其表达与患者性别和年龄无相关性(P>0.05),而与吸烟指数、肿瘤细胞分化、淋巴结转移、远处转移、TNM分期显著相关(P<0.05);98例患者5年随访期间77例患者死亡,10例患者存活,11例患者失访,5年生存率为11.49%。其中S100A6蛋白低表达患者5年累计生存率63.6%,中位生存时间68月;中表达患者5年累计生存率14.3%,中位生存时间45月;高表达患者5年累计生存率0%,中位生存时间29月,生存曲线显示S100A6不同表达状态患者生存率比较差异有统计学意义(P<0.05);COX回归模型分析显示,肿瘤分化、TNM分期和S100A6表达状态是影响肺腺癌患者预后的独立因素(P<0.05);S100A6蛋白表达对肺腺癌诊断灵敏度为84.69%,特异度为69.39%。结论 S100A6蛋白在肺腺癌组织中显著过表达,与肿瘤分期、分化、转移及预后密切相关。Objective To explore S100A6 protein expression in lung adenocarcinoma and its clinical significance. Methods Immunohistochemisty（ IHC） method was used to detect S100A6 protein expression in98 cases of lung adenocarcinoma tissues and their paired adjacent normal lung tissues. The interrelation of S100A6 protein expression and clinical and pathological characteristics was analyzed by statistics methods.Results The expression of S100A6 protein was higher in lung adenocarcinoma tissue than that in normal lung tissue（ P 0. 001）. The expression of S100A6 protein was not related to age and sex（ P 0. 05）,while is significantly related to smoking index,tumor differentiation,lymph node metastasis,distant metastasis and TNM stage（ P 0. 05）. During the 5- year follow-up period,77 patients of 98 patients died,10 patients survived,11 patients were lost,and the 5-year survival rate was 11.49%. The 5-year cumulative survival rate and the median survival time are respectively 63. 6% and 68 months in the patients with low expression of S100A6 protein; 14.3% and 45 months in the patients with moderate expression of S100A6 protein and 0% and29 months in the patients with high expression of S100A6 protein. Survival curve showed that the survival rate of patients with different S100A6 expression was statistically significant（ P 〈0.05）. Cox regression model revealed that the tumor differentiation,TNM stage and S100A6 protein expression were independent hazard factors oflung adenocarcinoma prognosis（ P 〈0.05）. The diagnostic sensitivity of the S100A6 protein expression level to lung adenocarcinoma was 84. 69% and the specificity was 69. 39%. Conclusions S100A6 protein is overexpressed in lung adenocarcinoma tissue and is closely related to the differentiation,stage,metastasis and prognosis of lung adenocarcinoma.

关 键 词：S100A6蛋白 肺腺癌 免疫组织化学 预后 

分 类 号：R563[医药卫生—呼吸系统] R734.2[医药卫生—内科学]