Effect of Echinacea Polysaccharide on Secretion of IkB-αmRNA by IEC-6 Injured by LPS  

作　　者：Qiumei SHI Yanying ZHANG Qinghui JIA Huiyuan ZHAO Minghe YU Guohui YU Jiwei An 

机构地区：[1]Hebei Normal University of Science&Technology,Hebei Provincial Key Laboratory of Preventive Veterinary Medicine,Changli 066600,China [2]Wenan Ani-mal Husbandry and Veterinary Bureau,Wenan 065800,China [3]Leting Animal Husbandry Bureau,Leting 063600,China

出　　处：《Agricultural Biotechnology》2017年第1期31-33,共3页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of China(31472230);Natural Science Foundation of Hebei Province(C2014407068);Fund from Science and Technology Department of Hebei Province(NO.14966610D)

摘　　要：[ Objective] This study was conducted to investigate the mechanism of Echinacea polysaccharide （EPS） in treatment of various bacterial infection and reduction of inflammation, so as to provide a theoretical basis for clinic application of EPS. [ Method ] Nuclear protein extracted from six groups, the normal control group, the simple lipopolysaccharide （LPS） group and the EPS （ with concentrations of 50,100,200 and 500 μg/ml, respectively） ＋ LPS groups was subjected to SDS-PAGE electrophoresis, and pIkB-α protein contents in the extracts were analyzed by Coomassie brilliant blue （CBB） staining and Western-Blot method. [ Result] The simple LPS group showed the highest pIkB-α protein level, and in the EPS concentration range of 0 -200 μg/ml, the expression level of pIkB-α pro- tein was improved with the increase of EPS concentration. [ Conclusion The expression level of pIkB-α protein was improved under the simulation of IEC-6 by LPS, while EPS could effectively inhibit the expression of pIkB-α protein. The expression level of pIkB-α was the lowest in the LPS ＋500 μg/ml EPS group.[ Objective] This study was conducted to investigate the mechanism of Echinacea polysaccharide （EPS） in treatment of various bacterial infection and reduction of inflammation, so as to provide a theoretical basis for clinic application of EPS. [ Method ] Nuclear protein extracted from six groups, the normal control group, the simple lipopolysaccharide （LPS） group and the EPS （ with concentrations of 50,100,200 and 500 μg/ml, respectively） ＋ LPS groups was subjected to SDS-PAGE electrophoresis, and pIkB-α protein contents in the extracts were analyzed by Coomassie brilliant blue （CBB） staining and Western-Blot method. [ Result] The simple LPS group showed the highest pIkB-α protein level, and in the EPS concentration range of 0 -200 μg/ml, the expression level of pIkB-α pro- tein was improved with the increase of EPS concentration. [ Conclusion The expression level of pIkB-α protein was improved under the simulation of IEC-6 by LPS, while EPS could effectively inhibit the expression of pIkB-α protein. The expression level of pIkB-α was the lowest in the LPS ＋500 μg/ml EPS group.

关 键 词：Intestinal epithelial cells LIPOPOLYSACCHARIDE Echinacea polysaccharide pIkB-α protein Expression Inhibition 

分 类 号：S853.7[农业科学—临床兽医学]