吉林省活禽市场外环境禽流感污染情况调查  

Environmental contamination of avian influenza virus in live poultry markets in Jilin province

作　　者：邓立权[1] 范明[1] 黄飚[1] 卢欣荣[1] 吴东林[2] 李静[2] DENG Li-quan FAN Ming HUANG Biao et al(Department of Infectious Disease Control and Prevention, Jilin Pro- vincial Center for Disease Control and Prevention, Changchun, Jilin Province 130062, China)

机构地区：[1]吉林省疾病预防控制中心传染病预防控制所,长春130062 [2]吉林省卫生监测检验中心

出　　处：《中国公共卫生》2017年第3期493-495,共3页Chinese Journal of Public Health

基　　金：吉林省自然科学基金(20140101031JC);吉林省重点科技攻关项目(20150204005SF)

摘　　要：目的目的了解吉林省活禽市场外环境禽流感病毒分布状况。方法于2014年3月1日—10日采集吉林省活禽市场外环境,共抽取370个采样点,每个点抽取50家活禽摊位采集外环境鸡笼涂抹、鸡粪和污水标本,采用实时荧光定量PCR法检测禽流感病毒FluA H5、H9和H7核酸。结果 370个采样点中58个(15.68%)检出阳性标本,各地区标本核酸阳性率有明显差异(P<0.05);共采集活禽市场外环境标本1 110份,分别为污水359份,粪便381份,笼具擦拭物370份,其中阳性94份,H9阳性60份,H5阳性30份,H5/H9混合阳性4份,未检测出H7阳性,不同类型标本的阳性检出率无统计学差异。结论吉林省活禽市场环境存在禽流感病毒的污染,主要型别为H9和H5亚型,未发现H7亚型。Objective To examine environmental contamination distribution of avian influenza virus in live poultry markets in Jilin province.Methods Hencoop swap specimens,chicken manure and sewage samples were collected with random sampling from 370 sampling sites at live poultry markets in 11 municipalities,prefectures,and counties across Jilin province during March of 2014 and detected for viral nucleic acid of avian influenza （H5,H9 and H7） with real-time fluorescence quantitative PCR.Results Of 1 110 samples detected,94 （8.47%） were avian influenza virus positive; of the positive samples,60 were positive for avian influenza H9,30 positive for H5,and 4 were positive for both H5 and H9; no positive sample for avian influenza H7 was detected.Conclusion Avian influenza virus contamination exists and H9 and H5 are predominant strains in the environment of live poultry markets in Jilin province.

关 键 词：活禽市场 禽流感 吉林省 

分 类 号：R511.7[医药卫生—内科学]