DNAzyme聚乙烯吡咯烷酮/Ru(bpy)3^2＋/SiO2纳米粒子相互作用及铅(Ⅱ)的电化学发光分析方法研究  

Investigation of interaction between DNAzyme and polyvinylpyrrolidone(PVP)/Silica/Ru(bpy)_3^(2+) nanoparticles for label-free electrogenerated chemiluminescent detecting Pb(Ⅱ)

作　　者：付文[1] 蔡林芳[1] 郭志慧[1] 郑行望[1] 

机构地区：[1]陕西省生命分析重点实验室,陕西师范大学化学化工学院,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2017年第2期49-55,共7页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金(21375085)

摘　　要：合成了一种对单、双链DNA具有不同亲和能力的纳米粒子PVP/Silica/Ru(bpy)_3^(2+)。实验结果发现,Pb(Ⅱ)能够诱导DNAzyme结构的变化,纳米粒子PVP/Silica/Ru(bpy)_3^(2+)在Nafion/碳纳米管修饰电极表面具有电化学发光特性,据此建立了一种检测铅离子的电化学发光新方法。该方法具有免标记DNA探针、无需固定化DNAzyme、灵敏度高等特点。在最佳实验条件下,该方法对铅离子的检测限达0.5nmol/L,实现了水样中Pb(Ⅱ)的电化学发光分析检测。The PVP/Silica/Ru （bpy）3^2＋ nanoparticles were synthesized, which show different af finities for singl duced by Pb（ Ⅱ the Nafion/carb nesence method e and double-stranded DNA. It was found that changes of DNAzyme structure in- ） resulted in the change in electrochemiluminesence properties of nanoparticles in for dete immobilized DNAzyme, ctmg and Pb（ high Ⅱ ） was developed. The this new electrochemiluminescence method is a label-free DNA probe r the optimal experimental conditi resented a detection limit of 0.5 m L, and was successfully applied for detecting Pb（ Ⅱ ） in water samples.

关 键 词：电化学发光 DNA酶 二氧化硅纳米粒子 联吡啶钌 Pb(Ⅱ) 

分 类 号：O657.1[理学—分析化学]