乙型肝炎病毒母婴垂直传播与乙肝病毒DNA载量的关系研究  

Relationship between vertical transmission of hepatitis B virus and hepatitis B virus DNA

作　　者：汤恋花 李晶[1] 彭忠秀 尹艳艳[1] 

机构地区：[1]广东省广州市第八人民医院妇产科,广东广州510000

出　　处：《中国医学工程》2017年第1期4-6,共3页China Medical Engineering

摘　　要：目的研究乙型肝炎感染产妇的乙肝病毒(HBV)DNA载量与母婴垂直传播之间的关系。方法用荧光定量PCR法对孕妇产前血清的HBV-DNA载量检测,按照载量分为两组,采用酶联免疫吸附实验(ELISA)对每组婴儿的脐带血进行检测。结果以5.0×10~2 copies/ml和1.0×10~6 copies/ml为HBV-DNA载量的分界点(其中5.0×10~2 copies/ml又为HBV阴性和阳性的分界点),按照HBV-DNA载量从小到大的顺序将选取的240名产妇分为3组,各组人数分别为120、53和67,各组的脐血HBV-DNA阳性率分别为4.17%(5/120)、11.32%(6/53)、35.82%(24/67)。HBV阴性和HBV阳性组间感染率比较差异有统计学意义(P<0.05),HBV阳性两组间感染率比较差异有统计学意义(P<0.05)。结论乙型肝炎感染孕妇的HBV-DNA载量越大,母婴垂直传播的概率越大。孕妇孕期建议定期监测HBV-DNA,如血液HBV-DNA≥1.0×10~6 copies/ml建议服用抗病毒药物治疗以减少母婴传播风险。【Objective】To study the relationship between vertical transmission of hepatitis B virus（HBV） from mother to child and DNA content of HBV.【Methods】The HBV-DNA of pregnant women was detected by polymerase chain reaction（PCR） and separated to two groups. The umbilical cord blood of each group of infants was tested by enzyme-linked immunosorbent assay（ELISA）.【Results】A total of 240 women were divided into three groups according to the order of HBV-DNA, which included 120, 53 and 67 women, respectively. The positive rates of HBV-DNA were 4.17%（5/120）, 11.32%（6/53）, 35.82%（24/67）, respectively. There was signifi cant difference in the infection rate between HBV negative and HBV positive group（P 0.05）, and the difference was statistically significant between the two HBV positive groups（P 0.05）.【Conclusion】The HBV-DNA content is higher, the probability of vertical transmission of the mother-infant is higher. We suggest that HBV-DNA should be regularly monitored in pregnant women. If blood HBV-DNA ≥1.0×106 copies/ml, the pregnant women should take antiviral drugs to reduce the risk of transmission.

关 键 词：乙型肝炎 母婴垂直传播 HBV-DNA载量 

分 类 号：R512.62[医药卫生—内科学]