冷蒿酚酸及其抗氧化防御酶活性对机械损伤的响应  

Responses of phenolic acid and defensive enzyme activities to mechanical damage in Artemisia frigida

作　　者：刘盟盟[1] 贾丽[1] 程路芸 张洪芹[1] 臧晓琳[1] 宝音陶格涛[2] 张汝民[1] 高岩[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 [2]内蒙古大学生命科学学院,呼和浩特010021

出　　处：《植物生态学报》2017年第2期219-230,共12页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金(31270756和31470704);国家重点基础研究发展计划(973计划)(2014CB138805);国家科技支撑计划项目(2011BAC07B01)

摘　　要：为了探讨放牧过程中冷蒿(Artemisia frigida)酚酸及其抗氧化酶活性对牲畜采食和践踏损伤的响应,该研究对盆栽冷蒿幼苗枝叶采用不同程度(轻度、中度和重度)机械损伤的方式模拟放牧强度,利用高效液相色谱技术测定了机械损伤处理6 h后冷蒿枝叶和根系9种酚酸含量的变化;同时对多酚氧化酶(PPO)、苯丙氨酸氨裂合酶(PAL)和抗氧化酶活性进行了测定,分析了酚酸含量与PPO和PAL活性之间的相关性。结果表明:轻度机械损伤可以诱导冷蒿枝叶和根系中PPO、PAL和抗氧化酶迅速做出响应,活性显著升高;随着机械损伤强度增加,冷蒿枝叶游离态咖啡酸、丁香酸、阿魏酸和肉桂酸含量显著增加,与对照相比中度损伤处理分别增加了150.4%、93.5%、154.4%和121.7%,与PAL活性呈正相关关系;游离态绿原酸和邻苯二酚含量减少,与对照相比重度损伤处理后降低了91.1%和69.3%,与PPO活性呈负相关关系;与对照相比重度损伤处理下没食子酸和原儿茶酸分别增加了280.6%和215.7%。随损伤强度增加,根系中9种游离态酚酸含量均显著增加,其含量均小于叶片。机械损伤导致冷蒿枝叶中游离态和结合态酚酸总量增加,根系中结合态酚酸减少。上述结果表明机械损伤首先诱导冷蒿体内应激防御系统中抗氧化酶和酚酸合成关键酶活性升高,然后促进抗氧化物质酚酸的积累,调节冷蒿植株内木质素合成以及醌类和单宁形成,增强冷蒿抗机械损伤能力和提高冷蒿的耐牧性。Aims The study aims at understanding the effects of feed intake and trample damage on the phenolic acid for- mation and antioxidant enzyme activities in Artemisia frigida, and elucidating the adaptive mechanisms in A. fri- gida to grazing in secondary metabolites and their related enzyme activities. Methods We analyzed the phenolic acid content and the activities of polyphenol oxidase （PPO）, phenylalanine ammonia-lyase （PAL） and protective enzymes in leaves and roots in A. frigida under three levels （light, moderate, and heavy） of manipulative grazing condition. The measurements of the 9 phenolic acid contents started after 6 h of the mechanical damage of the plants by using the high performance liquid chromatography （HPLC）, and the enzyme activities in leaves and roots were measured by a spectrophotometry method. Important findings The light damage treatment induced productions of PPO, PAL and significantly （p 〈 0.05） increased antioxidant enzyme activities in the leaves and roots ofA. frigida. The contents of PPO, PAL and anti- oxidant enzymes increased with increasing intensity of mechanical damage. Compared to the control, the content of free caffeic, syringic, ferulic and cinnamic acid in the leaves A. frigida were significantly elevated （p 〈 0.05） by 150.4%, 93.5%, 154.4% and 121.7%, respectively. They were significantly （p 〈 0.05） positively correlated with PAL activity in the moderate damage treatment. The content of free chlorogenic acid and catechol decreased by 91.1%, and 69.3%, respectively, compared with the control they had a negative correlation with PPO activity in the heavy damage treatment. The contents of gallic and protocatechuic acids increased （p 〈 0.05） by 280.6% and 215.7%, respectively, in the heavy damage treatment. With increasing intensity of mechanical damage, the content of 9 free phenolic acids significantly increased in roots but the increasing range was less than the one in leaves. Mechanical damage induced an increasing trend in the total a

关 键 词：冷蒿 机械损伤 酚酸 抗氧化酶 苯丙烷代谢 

分 类 号：Q948[生物学—植物学]