房颤患者心房肌细胞的原代培养与应用  

Primary Culture and Application of Atrial Myocytes from Patients with Atrial Fibrillation

作　　者：卞誉豪 张飞[1] 

机构地区：[1]深圳市南山区人民医院心胸外科,中国广东深圳518057 [2]广州医科大学研究生学院,中国广东广州511436

出　　处：《生命科学研究》2017年第1期51-54,共4页Life Science Research

基　　金：深圳市科创委2013年度知识创新计划-基础研究项目(JCYJ20130322154529556);深圳市南山区2012年度科技计划项目(南科研卫2012003号)

摘　　要：探索成功培养房颤(atrial fibrillation,AF)患者原代心房肌细胞的方法,能够为房颤发病机制的研究奠定实验基础。取心胸外科行迷宫手术患者的左心耳,利用I型胶原蛋白酶消化法培养房颤患者的原代心房肌细胞,通过免疫组化进行鉴定,同时对培养的心房肌细胞进行初步研究。与动物实验相反,房颤患者心房肌细胞缝隙连接蛋白40(connexin40,Cx40)及Kv1.5钾离子通道蛋白的表达量均降低。由此可见直接研究成人房颤患者的心房肌细胞具有更高的可靠性,同时也证明了房颤患者心房肌细胞的培养是研究其发病机制的基础。Exploring the methods for primary culture of atrial myocytes from patients with atrial fibrillation （AF） can provide practical methods for research about AF. Here, the left atrial appendage specimens were obtained from patients with AF during cardiac surgery. Then, primary atrial myocytes, which were isolated from the specimens by collagenase digestion method, were identified through immunohistochemistry and analyzed simultaneously. In contrast with some animal experiments, low expressions of connexin40 （Cx40） and Kv1.5 channel protein were found in AF patients. The results showed that studying atrial myocytes of AF patients will be more reliable. Meanwhile, atrial myocyte culture from AF patients is the basis for research on the pathogenesis of AF.

关 键 词：心房颤动(AF) 心房肌细胞 细胞培养 缝隙连接蛋白40(Cx40) 钾离子通道(Kv1.5) 

分 类 号：R318.11[医药卫生—生物医学工程] R541.75[医药卫生—基础医学]