高效液相色谱法测定防晒化妆品中水杨酸酯类紫外线吸收剂的含量  

HPLC Determination of Salicylic Acid Ester UV Absorbers in Sunscreen Cosmetics

作　　者：池琴[1] 钱春苹[1] 王魁[1] 

机构地区：[1]鄂尔多斯职业学院,鄂尔多斯017000

出　　处：《理化检验（化学分册）》2017年第3期289-294,共6页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用高效液相色谱法同时测定防晒化妆品中8种水杨酸酯类紫外线吸收剂(水杨酸乙基己酯、水杨酸三甲环己酯、水杨酸辛酯、水杨酸苯酯、水杨酸异十六酯、水杨酸己酯、水杨酸乙二醇酯和水杨酸苄酯)的含量。1.000 0g样品经5mL乙酸乙酯于20℃超声萃取10min,萃取液在Agilent ZORBAX Eclipse XDB-C_(18)色谱柱上分离,以甲醇-四氢呋喃-0.1%(质量分数)甲酸溶液为流动相进行梯度洗脱,紫外检测波长为340nm。8种水杨酸酯类紫外线吸收剂质量浓度在5.0~100.0mg·L^(-1)内与峰面积呈线性关系,检出限(3S/N)在0.5~2.5mg·kg^(-1)之间;加标回收率在90.6%~98.8%之间,测定值的相对标准偏差(n=6)在1.4%~3.6%之间。HPLC was applied to the simultaneous determination of 8 kinds of salicylic acid ester UV absorbers, including ethylhexyl salicylate, homosalate, octyl salicylate, phenyl salicylate, salicylic acid, salicylic acid hexyl ester with sixteen salicylic acid, ethylene glycol and benzyl salicylate, in sunscreen cosmetics. 1. 000 0 g of sample was extracted by ultrasonic with 5 mL of ethyl acetate at 20 ℃ for 10 rain, and the extract was separated on an Agilent ZORBAX Eclipse XDB-C18 chromatographic column with a mixture of methanol, tetrahydrofuran, and 0. 1% （w） formic acid solution as mobile phase for gradient elution. UV detection was made at the wavelength of 340 nrn. Linear relationships were found between peak areas and mass concentrations of the 8 kinds of salicylic acid ester UV absorbers in the range of 5. 0--100. 0 mg·L^-1, with detection limits （3S/N） in the range of 0. 5- 2.5 mg· kg^-1. Recovery rates obtained by standard addition method were in the range of 90. 6%- 98. 8%, and RSDs （n=6） were in the range of 1.40%-3.6%.

关 键 词：高效液相色谱法 紫外吸收剂 水杨酸酯类 防晒化妆品 

分 类 号：O657.7[理学—分析化学]