高效液相色谱-电感耦合等离子体质谱法测定禽类生物样品中7种砷形态  

Determination of Seven Arsenic Species in Poultry Biological Samples by HPLC-ICP-MS

作　　者：刘崴[1] 胡俊栋[1] 杨红霞[1] 李冰[1] 

机构地区：[1]国家地质实验测试中心,北京100037

出　　处：《理化检验（化学分册）》2017年第3期299-304,共6页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(41301565);中国地质科学院基本科研业务费项目(YYWF201620);中国地质大调查项目(12120113015400)

摘　　要：采用高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)联用技术测定禽类生物样品中砷甜菜碱(AsB)、二甲基砷(DMA)、As(Ⅲ)、一甲基砷(MMA)、As(Ⅴ)、阿散酸(ASA)和硝苯砷酸(NPAA)等7种砷形态。以人工胃液超声振荡法提取禽类生物样品中的砷,采用Dionex IonPacTM AS19阴离子交换柱分离7种砷形态,并用ICP-MS测定其含量。各砷形态的质量浓度在一定范围内与峰面积呈线性关系,检出限(3s,以砷计)在0.070~0.099μg·L^(-1)之间。加标回收率在74.0%~112%之间,测定值的相对标准偏差(n=6)在2.0%~5.9%之间。采用该方法分析矿区禽类生物样品,各砷形态含量的测定值之和与ICP-MS测得的总砷含量基本一致。HPLC-ICP-MS was applied to the determination of seven arsenic species, including arsenobetaine, dimethyl arsenic, As（ Ⅲ ）, monomethyl arsenic, As（Ⅴ）, arsanilic acid and nitrobenzene arsenic acid, in poultry biological samples. Arsenic in samples was extracted with artificial gastric juice by ultrasonic oscillation method, and the seven arsenic species were separated on a Dionex IonPacTM AS19 ion exchange column and determined by ICP-MS. Linear relationships were found between peak areas and mass concentrations of the seven arsenic speues in defined ranges, with detection limits （3s） in the range of 0. 070-0. 099 μg·L^-1. Recovery rates obtained by standard addition method were in the range of 74. 0%- 112%, and RSDs （n= 6） were in the range of 2. 0%- 5.9%. The method was used to analyze poultry biological samples in mining area, giving the sum of contents of seven arsenic species in agreement with the contents of total arsenic determined by ICP-MS.

关 键 词：高效液相色谱-电感耦合等离子体质谱法 砷形态 禽类生物样品 人工胃液 

分 类 号：O657[理学—分析化学]