盐胁迫下巴西蕉幼苗CaM含量的变化  

The Change of CaM content in Brazil Banana Seedlings under Salt Stress

作　　者：王文昌[1,2] 周双云[2] 乔飞[3] 吉福桑 李元元[1] 李新国[1] WANG Wenchang ZHOU Shuangyun QIAO Fei JI Fusang LI Yuanyuan LI Xinguo(College of Horticulture and Landscape Architecture,HainanUniversity, Haikou, Hainan 570228 Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007 Tropical Crops Genetic Resource Institute, CATAS, Danzhou, Hainan 571737)

机构地区：[1]海南大学园艺园林学院,海南海口570228 [2]广西农业科学院园艺研究所,广西南宁530007 [3]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《热带农业科学》2017年第2期12-15,共4页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金(No.31260462);海南大学中西部计划学科建设项目(No.ZXBJH-XK008)

摘　　要：研究巴西蕉幼苗在60 mmol/L Na Cl人工模拟的盐胁迫0、4、8、12、24和48 h不同时间下,通过增加外源CaCl_2和钙离子螯合剂EGTA处理后,测定其叶片和根的钙调蛋白(CaM)含量的变化。结果表明,与对照处理相比,在NaCl胁迫过程中,叶片CaM含量在4、8、12 h显著增加而在24和48 h显著减少;根的CaM含量则是在4、12、24 h显著增加,在8 h显著降低。在NaCl胁迫中添加CaCl_2处理能显著提高CaM含量,而在NaCl胁迫中添加EGTA处理能显著降低CaM含量。以上说明,通过CaM的含量变化,钙信号系统参与香蕉的耐盐调控。Brazil banana seedlings were treated with salt under 60 mmol/L NaCl salt stress environment at 0, 4, 8, 12, 24 or 48 h and added with exogenous Ca Cl2 and calcium chelator EGTA to determine their CaM content in roots and leaves.The results showed that the CaM content of the leaves significantly increased at 4 h, 8 h or 12 h and significantly decreased at 24 h or 48 h under Na Cl stress as compared to the control.The CaM content of the roots significantly increased at 4 h, 12 h or 24 h, and significantly decreased at 8 h.The CaM content could significantly increased by adding CaCl_2 under the NaCl stress,and significantly decreased by adding EGTA under the Na Cl stress.This indicates that calcium signaling system may be involved in the salt regulation of banana through the change of CaM content.

关 键 词：巴西蕉幼苗 盐胁迫 CaM含量 

分 类 号：S668.1[农业科学—果树学]