一株深海细菌Idiomarina sp.406-6的微生物学特征分析  

Microbiological Characterization of Strain Idiomarina sp.406-6 Isolated from Deep-sea

作　　者：陈国忠[1] 潘云军[1] 王文泽[1] 林慧媛 张燕娇[1] CHEN Guozhong PAN Yunjun WANG Wenze LIN Huiyuan ZHANG Yanjiao(Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China)

机构地区：[1]青岛农业大学,山东省应用真菌重点实验室,山东青岛266109

出　　处：《青岛农业大学学报（自然科学版）》2017年第1期27-33,共7页Journal of Qingdao Agricultural University(Natural Science)

基　　金：国家自然科学基金项目(31300005);青岛农业大学高层次人才科研基金(6631113308)

摘　　要：从印度洋海区水深为4 517m的深海泥样中筛选到一株细菌,菌株号为406-6。通过形态学特征和生理生化特征分析表明,菌株406-6为革兰氏阴性菌,细胞呈稍微弯曲的棒状。菌落为乳白色,圆形。菌株406-6生长需要NaCl,62℃时生长微弱,能利用小分子的甘氨酸作为唯一碳源生长,但不能利用L-阿拉伯糖、D-木糖、D-葡萄糖、D-半乳糖、D-甘露糖、蔗糖、D-甘露醇、D-海藻糖和D-纤维二糖作为唯一碳源。能水解酪素、明胶、脱脂奶粉、Tween 40和Tween 60,但是不能水解淀粉、Tween 20和Tween 80。16SrRNA基因序列比对分析表明,菌株406-6与Idiomarina属的I.fontislapidosi F23~T相似性最高(98.0%),和其他属的相似性均低于94.0%。Neighbor-joining系统树中,菌株406-6和Idiomarina属内的菌种相聚成簇。基于以上微生物学特征分析表明,406-6为Idiomarina属内的一个潜在的细菌新物种,该研究丰富了Idiomarina属的物种多样性,为微生物资源的开发利用奠定基础。A bacterium, designated 406--6, was isolated from sediment of the Indian Ocean at a water depth of 4517 m. Based on the results of the morphological and physiological--biochemical characterization of strain 406-- 6, it is Gram--negative, straight or slightly curved rods. Colonies are white and circular. Strain 406--6 did not grow without NaCl, but could slightly grow at 62℃. Strain 406--6 could utilize small molecules of glycine as sole carbon sources for growth, but not L-- arabinose, D-- xylose, D-- glucose, D-- galactose, D-- mannose, sucrose, D--mannitol, D--trehalose and D--cellobiose. Hydrolyses casein, gelatin, skimmed milk, Tween 40 and Tween 60, but not starch, Tween 20 and Tween 80. Comparative analysis of the 16S rRNA gene sequence showed that strain 406--6 had the highest 16S rRNA gene sequence similarity to the type strain of Idiomarina fontislapidosi F23T（98.0%）, and less than 94.0% sequence similarity to type strains of other known genus. In the neighbor-- joining tree, strain 406--6 and the type strains of Idiomarina formed a coherent branch. Based on the results of the microbiological characterization of strain 406-- 6, it represents a potentially novel species of the genus Idiomar- ina. This study enriches the species diversity of the genus Idiomarina, and lays the foundation for the development and utilization of microbial resources.

关 键 词：深海 Idiomarina sp. 微生物学特征 

分 类 号：Q935[生物学—微生物学]