UPLC-MS/MS研究新人参二醇大鼠血浆蛋白结合率  

Plasma Protein Binding Characteristics of Neopanaxadiol in Rats by UPLC-MS/MS

作　　者：耿聪[1] 吴迪[2] 吕慧怡[1] 李玉中[1] 施维维[1] 刘永娥[1] 尹建元[2] 

机构地区：[1]大连医科大学附属第二医院,辽宁大连116023 [2]吉林大学药学院,长春130021

出　　处：《中国药学杂志》2017年第5期404-408,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(31070308);辽宁省教育厅科学研究项目(L2015141)

摘　　要：目的建立测定大鼠血浆中新人参二醇(NPD)浓度的UPLC-MS/MS检测方法,研究NPD的大鼠血浆蛋白结合率。方法在体外采用平衡透析法模拟NPD体内与血浆蛋白结合的过程,通过UPLC-MS/MS分析测定不同浓度NPD在透析袋内血浆中和透析外缓冲液中的浓度,计算NPD的大鼠血浆蛋白结合率。结果 NPD在0.05~8μg·mL^(-1)内呈现良好的线性关系,质控样品的日内和日间精密度(RSD)均小于15%,方法回收率均高于79.37%;NPD在低、中、高3个浓度水平下的大鼠血浆蛋白结合率分别为(86.55±4.50)%、(76.50±2.61)%和(78.25±1.32)%,各浓度间的结合率数据无显著性差异(P>0.05)。结论 NPD在大鼠体内主要以与血浆蛋白结合的形式存在。OBJECTIVE To develop an UPLC-MS/MS method for the determination of neopanaxadiol （NPD） in rat plasma sample, and to investigate plasma protein binding rate of NPD in rats. METHODS Under three different concentrations, equilibrium dialysis method was utilized to imitate the binding process between NPD and plasma protein. The concentration of NPD in and out of the dialysis membrane was determined by UPLC-MS/MS and the plasma protein binding rate of NPD were calculated. RESULTS Excel- lent linearity was found between 0. 05 - 8μg·m L-1. Intra- and inter-day precision values （RSD） of QC samples were both below 15% and the extraction recoveries of NPD from biological matrices were better than 79.37%. The plasma protein binding rates of NPD were （ 86. 55 ± 4. 50） % , （76. 50 ± 2. 61 ） % and （78.25 ± 1.32） % at low, middle and high concentrations, respectively. There was no significant difference among three concentrations. CONCLUSION These results indicate the high plasma protein binding rates of NPD in plasma in combination mode.

关 键 词：超高效液相色谱-串联质谱法 新人参二醇 血浆蛋白结合率 平衡透析法 

分 类 号：R969[医药卫生—药理学]