离体培养条件下GA对烟草胚柄PCD的诱导研究  

Suspensor PCD Induced by GA in Nicotiana tabacum Under In Vitro Culture

作　　者：罗岸[1] 陈克强[2] LUO Ani CHEN Ke-qiang(College of Life Science, Yangtze University, Jingzhou 434023 College of Life Science, Wuhan University, Wuhan 430072)

机构地区：[1]长江大学生命科学学院,荆州434023 [2]武汉大学生命科学学院,武汉430072

出　　处：《生物技术通报》2017年第3期71-77,共7页Biotechnology Bulletin

基　　金：长江大学自然科学基金项目(2014NSFY023)

摘　　要：细胞程序性死亡(PCD)对于植物的正常生长至关重要,涉及整个生命周期中的诸多发育事件,其中植物激素是植物细胞PCD的一种重要调控因子。本研究建立了有效的烟草胚珠和胚胎离体培养体系。通过设置不同的赤霉素(GA)浓度梯度和不同的培养天数进行比较,认为100μmol/L的GA浓度和不超过3 d的培养时间对于研究比较合适。胚胎离体培养表明外源GA能够有效诱导烟草胚柄细胞PCD的发生;且随着GA浓度的增加,胚柄细胞PCD的比例会逐步上升。但过高的GA浓度和过长的培养时间也会导致胚体细胞出现异常的PCD现象。Programmed cell death （ PCD ） is ubiquitous in the entire developmental process of plant, and hormones are a key regulating factor to PCD in plant. To investigate the role of GA in the suspensor PCD, systems of in vitro culture of the ovule and embryo were well established. By setting different concentrations of GA and culture time, 100 μmol/L GA was appropriate and embryos may not be cultured for more than 3 days. The suspensor PCD was efficiently induced by exogenous GA during in vitro culture of embryo. As the concentration of GA increased, the proportion of PCD in suspensor cells gradually raised. Howerver, excessively high concentration of GA and long culture time would also induce abnormal PCD in porper ceils.

关 键 词：胚柄细胞 PCD 赤霉素 离体培养 

分 类 号：Q944.4[生物学—植物学]