内生真菌对油樟挥发油积累及生理生化特性的影响  

Effect of Endophytic Fungi on Iits Essential Oil Accumulation and Physiological-biochemical Characteristic of Cinnamomum longepaniculatum

作　　者：严宽[1,2] 陈放[1] 魏琴[2] 冯瑞章[2] 周万海[2] 周敏[2] YAN Kuan CHEN Fang WEI Qin FENG Rui-zhang ZHOU Wan-hal ZHOU Min(College of Life Science, Sichuan University, Chengdu 610044 College of Life Science and Food Engineering, Yibin University, Yibin 644000)

机构地区：[1]四川大学生命科学学院,成都610044 [2]宜宾学院生命科学与食品工程学院,宜宾644000

出　　处：《生物技术通报》2017年第3期138-143,共6页Biotechnology Bulletin

基　　金：四川省教育厅项目(14TD0031);四川省大学生创新项目(201510641016);四川省高校重点实验室项目(2015XLZ001)

摘　　要：旨在研究内生真菌对油樟挥发油积累及生理生化特性的影响。以4株内生真菌(2J1、3J1、5J2、YB)作为材料,将内生真菌孢子悬浮液均匀喷洒在油樟叶片上进行处理后,提取油樟叶片中的挥发油,利用GC-MS测定挥发油中1,8-桉叶油素、α-松油醇的量。结果显示,采用2J1和3J1两种内生真菌对油樟进行处理,在处理21 d时挥发油含量达到最大值,其主要成分1,8-桉叶油素分别比对照高出201.53%、174.47%,α-松油醇高出364.14%、307.29%,且过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MOD)的含量有显著提高;由5J2和YB处理的油樟挥发油含量、POD与CAT活性以及MOD含量与对照相比差异均不显著。采用2J1和3J1处理油樟植株,能有效提高油樟挥发油的含量。The aim of this study was to examine the effects of endophytic fungi on the accumulation and physiological-biochemical characteristics of essential oil in Cinnamomum longepaniculatum. The essential oil of C. longepaniculatum was extracted after spraying four endophytic fungi spore suspension （2J1, 3J1, 5J2, YB ） on the leaves separately, and the content of 1, 8-cineole and α-terpineol was determined by GC-MS. The results showed that the content of essential oil reached a peak at 21 d after treatment with 2J1 and 3J1, and the contents of 1, 8-cineole increased by 201.53% and 174.47% compared with controls, and tt-terpineol increased by 364.14% and 307.29% as well, respectively. Furthermore, the peroxidase （ POD ） content, catalase （ CAT ） activity, and malondialdehyde （ MOD ） content of suspension cells increased significantly. No significant difference was detected between C. longepaniculatum treated with 5J2 and the control group in terms of essential oil accumulation, CAT and POD activity, and MOD contents, nor did YB. Consequently, treatment with 2J1 and 3J 1 could significantly increase the accumulation of essential oil in C. longepaniculatum.

关 键 词：内生真菌 油樟 挥发油 生理生化特性 

分 类 号：S792.23[农业科学—林木遗传育种]