OrexinA、OrexinB和AMH受体在性腺轴的分布及其生殖生物学意义的研究  

Study on the distribution of orexin and anti-mullerian hormone receptors in the hypothalamic-pituitary-testicular axis and their reproductive biological significance

作　　者：海米提·阿布都力木[1] 李艳[1] 郭琼[1] 荣小灵[2] 李甜[1] 阿地力江·伊明[3] Haimiti.Abudulimu Li Yan Guo Qiong Rong Xiaoling Li Tian Adilijiang.Yiming(Department of Histology and Embryology, Urumqi 830011, China Clinical Laboratory of the Second Affiliated Hospital, Xinjiang Medical University Department of Anatomy, Xinjiang Medical University)

机构地区：[1]新疆医科大学基础医学院组胚教研室,乌鲁木齐830011 [2]新疆医科大学第二附属医院检验科 [3]新疆医科大学基础医学院人体解剖学研室

出　　处：《中国男科学杂志》2016年第11期3-8,共6页Chinese Journal of Andrology

摘　　要：目的研究食欲素受体1(OX1R)、食欲素受体2(OX2R)、抗中肾旁管激素受体Ⅱ(AMHR2)在下丘脑-垂体-睾丸轴中分布与细胞定位,探讨其对生精功能的调节机制。方法用免疫组织化学法检测OX1R,OX2R和AMHR2受体在性未成熟和性成熟SD大鼠的下丘脑、垂体、睾丸表达情况。结果性未成熟组:OX1R,OX2R和AMHR2受体在下丘脑室周带、内侧带、外侧带神经元细胞质及细胞膜表达阳性,在腺垂体少数细胞细胞质阳性,在睾丸只有间质细胞细胞膜及细胞质表达阳性。性成熟组:OX1R、OX2R和AMHR2受体在下丘脑室周带、内侧带、外侧带神经元细胞质及细胞膜表达阳性,在腺垂体少数细胞细胞质阳性,在睾丸间质细胞和精原细胞细胞膜及细胞质表达阳性。结论 OX1R、OX2R和AMHR2受体在性成熟与未成熟的大鼠下丘脑、脑垂体表述无差异,在睾丸分布上具有不同,上述3种受体可能参与性腺轴调节精原细胞分化功能。Objective To study the distribution of orexin receptor 1 （OX1R）, orexin receptor2 （OX2R）, antimullerian hormone receptor II （AMHR H ） in hypothalamus, pituitary and testis of immature and mature male SD rats. ＂Methods Immunohistochemical stain was applied to detect the expressions of OX1R, OX2R, and AMHR II Receptors in hypothalamus ,pituitary and testis of the both rats. Results OX1R, OX2R, and AMHR II receptors positively expressed at the neuron cell membrane and cytoplasm of periventricular zone, medial zone and lateral zone of hypothalamus in the both rats; there was also some positive cells in adenohypophysis of the both; the distribution of three receptors was obviously differences in the testis ,there were positive expressions of three receptors at cytoplasm and cell membrane of leydig cells in testis of immature rats, but there were positive expression of three receptors at cytoplasm and cell membrane of leydig cells and also spermatogonium in testis of mature rats. Conclusion Higher expression of three receptors were found in the hypothalamus-Pituitary-Testistular axis of the rats, indicating that orexin and anti-mullerian hormones may be associated with spermatogonium differentiation of male reproductive system.

关 键 词：食欲素受体 中肾旁管激素受体 大鼠 SPRAGUE-DAWLEY 莱迪希细胞 精原细胞 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]