养精胶囊通过调控StAR启动子促进睾酮合成的研究  

Study on Yangjing Capsule promoting testosterone synthesis through regulating StAR promoter

作　　者：孙大林[1] 陈广辉[2] 金保方[1] 蔡滨[1] 邓伟民[1] Sun Dalin Chen Guanghui Jin Baofang Cai Bin Deng Weimin(Andrology Department of Integrative Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, China Department of Andrology, Hebei Province Hospital of Traditional Chinese Medicine)

机构地区：[1]东南大学附属中大医院中西医结合男科,南京210009 [2]河北省中医院男科

出　　处：《中国男科学杂志》2016年第11期25-28,共4页Chinese Journal of Andrology

基　　金：国家自然科学青年基金(No.81403402);国家自然科学基金(No.81273760;81473678)

摘　　要：目的探讨养精胶囊促进睾丸间质(Leydig)细胞合成睾酮(T)功能的具体作用机制。方法在Leydig细胞(MLTC-1细胞系)中加入不同剂量养精胶囊提取液24 h后,用化学发光法检测细胞上清T浓度;用免疫荧光显微镜分析类固醇快速调节蛋白(StAR)的表达情况;用RT-PCR及Western blot法测StAR mRNA及蛋白质的表达;用双荧光素酶报告基因实验检测StAR启动子的活性。结果低、中、高剂量养精胶囊提取液作用24 h后,均可以促进MLTC-1细胞合成睾酮的功能;养精胶囊提取液可以促进MLTC-1细胞StAR免疫荧光的表达;养精胶囊提取液可以促进MLTC-1细胞StAR mRNA和蛋白质的表达;养精胶囊提取液可以提高MLTC-1细胞中StAR启动子的活性。结论养精胶囊能通过调控Leydig细胞中StAR启动子的活性,促进StAR mRNA和蛋白的表达,进而提高睾酮合成的功能。Objective To explore the mechanism of Yangjing Capsule increasing the synthesis of testosterone in Leydig cells. Methods Leydig cells （MLTC-1 cell line） were incubated for 24 h with different dose of Yangjing Capsule extract. T in culture supernatant was determined using chemiluminescence. The expression of StAR was detected by immunofluorescence microscopy. RT-PCR and western blot were used to detect the expression of StAR mRNA and protein. The activity of StAR promoter was detected by double luciferase reporter assay. Results After 24 h, the low, medium and high dose of Yangjing capsule extract all promoted the synthesis of testosterone in MLTC-1 cells. The Yangjing Capsule extract forced the immunofluorescence expression of STAR, and also upregulated the expression of StAR mRNA and protein in MLTC-1 cells. Simultaneously, Yangjing Capsule extract enhanced the activity of StAR promoter in MLTC-1 cells. Conclusion Yangjing Capsule can strengthen the activity of StAR promoter and increase the expression of StAR in Leydig cells, and ultimately improve the function of testosterone synthesis.

关 键 词：养精胶囊 莱迪希细胞 睾酮 

分 类 号：R698.2[医药卫生—泌尿科学]