Olsen法测定土壤中有效磷的方法优化及检测研究  

The Methods Optimization and Detection of the methods Olsen Determinate Soil Available Phosphorus

作　　者：张飞龙[1] Zhang feilong(Institutions of Agricultural Product Quality Standrad and Testing Research, Tibet Academy and Animal Husbandry Sciences, Lhasa 850032, Chin)

机构地区：[1]西藏自治区农牧科学院农业质量标准与检测研究所,西藏拉萨850032

出　　处：《西藏农业科技》2016年第4期33-37,共5页Tibet Journal of Agricultural Sciences

摘　　要：土壤有效磷含量的测定最常用的方法是碳酸氢钠浸提-钼锑抗比色法(Olsen法)。该方法测定过程中有机质对测定值影响比较大,一般采用无磷活性炭吸附的办法去除有机质的干扰。据《土壤分析技术规范》注释,测定时可建议采用紫外可见分光光度计于波长880nm处比色,此波长处对浸出的有机质已基本无吸收,浸提时可不加活性炭。本实验拟探讨这两种方法对土壤有效磷测定值的影响。实验结果表明,在紫外波长880nm处对土壤有机质背景的干扰能够有效的去除,测定值结果相较与传统的活性炭法没有显著性差异,测定过程具有准确、简单、快捷、低成本的优势。故采用紫外可见分光光度计于波长880nm处比色能够准确的测定土壤有效磷。The most commonly used method of the Determination of soil available phosphor- us content is sodium bicarbonate extraction Mo--Sb colorimetry （Olsen method）. During the de- termination, Organic matter impact on the measured value is larger, Generally no phosphorus ad- sorption were used to remove the interference of organic matter According to the annotation of 《the technical specification for soil analysis》, It may recommend using ultraviolet-- uisible spec- trophotometer at the wavelength of 880nm at the determination of colorimetrie,The wavelengthsin the leaching of organic matter has been basically no absorption, extraction without activated carbon. This study intends to explore the two methods of measurements of available in soil. Ex- perimental results show that the ultraviolet wavelength of 880 nm to effectively remove the inter- ference of background of soil organic matter,The measurements results, There are no significant difference in comparison with the traditional method of activated carbon, the measurement process is accurate, simple, fast, low--cost advantages. The ultraviolet--uisible spectrophotom-- eter is adopted 880 nm wavelengths of colorto the accurate determination of soil effective phos- phorus.

关 键 词：有效磷 有机质 紫外可见分光光度计 无磷活性炭 

分 类 号：S153.61[农业科学—土壤学]