高效液相色谱法测定草地早熟禾4种内源激素的方法  

A Detection Method of Four Endogenous Hormones in Poa pratensis L.by HPLC

作　　者：张咏梅[1] 马晖玲[2] 张锁科 唐云智[2] ZHANG Yong mei MA Hui-ling ZHANG Suo-ke TANG Yun-zhi(Instrumental Research and Analysis Center, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China Pratacultural college, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China)

机构地区：[1]甘肃农业大学研究测试中心,甘肃兰州730070 [2]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《草地学报》2016年第6期1384-1387,共4页Acta Agrestia Sinica

基　　金：国家自然科学基金(31160482)资助

摘　　要：建立了高效液相色谱法测定草地早熟禾中玉米素（ZT）、吲哚乙酸（IAA）、脱落酸（ABA）和赤霉素（GA3）的方法。样品用80%甲醇水溶液冷浸提取。色谱柱为Extend-C_8（4.6×150mm,5μm）色谱柱,流速为1mL·min-1,进样量均为20μL。前3种激素的流动相为甲醇和0.6%冰乙酸梯度洗脱;检测波长为254nm。GA3流动相为乙腈：0.6%冰乙酸=60︰40等度洗脱;检测波长为208nm。4种激素在一定检测范围内具有良好的线性关系,相关系数均达0.99以上;平均回收率为83.78%～99.09%;相对标准偏差均小于5%。结果表明：该方法灵敏度、精密度和准确度好,可以满足草地早熟禾ZT,IAA,ABA和GA34种激素的检测。A method has been developed for the detection of the contents of zeatin（ZT）,indoleacetic acid（IAA）,abscisic acid（ABA）and gibberellins（GAs）in Poa pratensis L.by HPLC（High Performance Liquid Chromatography）.After being extracted with 80% methanol from samples,four endogenous hormones were separated on a Extend-C8column（150×4.6mm,5μm）at flow velocity of 1mL·min-1 and injection volume of 20μL.ZT,IAA and ABA were detected at wavelength of 254 nm using methanol and0.6% acetic acid as mobile phase with gradient elution,While GA3 were detected at wavelength of 208 nm using acetonitrile and 0.6% acetic acid（60∶40）as mobile phase.There were good linear correlations between the concentrations and peak areas of the four hormones above the correlation coefficients of 0.99.The average recoveries were around 83.78%~99.09%,and relative standard deviations were below 5%.The results suggested that the method can be used to detect the contents of ZT,IAA,ABA and GA3 in Poa pratensis L.with good sensitivity,precision and accuracy.

关 键 词：草地早熟禾 内源激素 高效液相色谱 

分 类 号：S688.4[农业科学—观赏园艺]