头针对脑缺血再灌注大鼠脑组织中组织金属蛋白酶抑制因子-1表达调控机制的动态研究  

A dynamic study of scalp acupuncture on regulation of expression of brain TIMP-1 in rats with cerebral ischemia reperfusion

作　　者：于学平[1] 孙晓伟[1] 邹伟[1] 张鑫[1] YU Xueping SUN Xiaowei ZOU Wei et al(The First Hospital Affiliated to Heilongjiang University of Chinese Medicine, Harbin, 150040)

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040

出　　处：《中国康复医学杂志》2017年第3期283-287,共5页Chinese Journal of Rehabilitation Medicine

基　　金：哈尔滨市科技局科技创新人才项目(优秀学科带头人)(2014RFXGJ030)

摘　　要：目的:动态观察头针对脑缺血再灌注大鼠脑组织中组织金属蛋白酶抑制因子-1(TIMP-1)表达的影响,以探讨头针抗脑缺血再灌注损伤的可能作用机制。方法:将SD雄性大鼠130只随机分为假手术组、模型组和针刺组。采用改良的Zea Longa线栓法制作脑缺血再灌注模型,针刺组选取百会透刺曲鬓穴进行手捻针治疗。应用免疫组化和免疫印迹检测方法,比较三组不同时点脑组织TIMP-1阳性表达与含量的变化。结果:针刺组各时间点TIMP-1较模型组均有升高趋势,再灌注4h、12h、24h、48h与模型组比较均无显著性差异(P>0.05);再灌注72h仍维持在较高水平,与模型组相比差异具有显著性意义(P<0.05)。结论:早期头针治疗能够上调缺血侧脑组织TIMP-1的表达,并随针刺治疗时间的延长,其疗效更加显著。上调TIMP-1以抑制MMP-9活性或阻断MMP-9的合成,可能是头针拮抗脑缺血再灌注损伤的作用机制之一。Objective： To observe the effect of scalp acupuncture on the expression of tissue inhibitor of metalloproteinases-1（TIMP-1）in the rats with cerebral ischemia reperfusion for exploring the interaction mechanisms. Method： One hundred and thirty male SD rats were randomly divided into three groups： sham operation group, model group, and scalp acupuncture group. Cerebral ischemia/reperfusion model was induced by modified Longa＇s method. Scalp acupuncture group was treated with Baihui-Qubin acupuncture. The positive expression and protein content of TIMP-1 were detected by immunohistochemistry and western blot. Result： Compared with model group, the expression of TIMP-1 in the scalp acupuncture group was on increasing curve, but there was no significant difference between the two groups after 4h, 12h, 24h, 48h reperfusion（all P＞0.05）. The expression of TIMP-1 in the scalp acupuncture was higher than that in the model group after 72h reperfusion（P＜0.05）. Conclusion： Scalp-acupuncture can effectively up-regulate TIMP-1 expression in the rats after acute cerebral ischemia/reperfusion, which may be more obvious as the treatment time goes on. Increased TIMP-1 can restrain the activity of MMP-9 or block up the synthesize of MMP-9, which may ameliorate cerebral ischemia reperfusion injury.

关 键 词：头针 脑缺血再灌注 组织金属蛋白酶抑制因子-1 血脑屏障 

分 类 号：R245[医药卫生—针灸推拿学]