靶向沉默HDAC6对乳鼠心房肌成纤维细胞活化增殖的作用  

The effect of HDAC6 silencing on proliferation in neonatal rat atrial fibroblasts

作　　者：曹炜[1] 石开虎[1] 陶辉[1] 施鹏[1] 吴超[1] 宣海洋[1] 沙纪名[1] 占红英[1] 

机构地区：[1]安徽医科大学第二附属医院心胸外科,安徽医科大学心血管病研究中心,合肥230601

出　　处：《安徽医科大学学报》2017年第3期356-359,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校自然科学研究项目(编号:KJ2015A320);安徽省科技攻关项目(编号:1501041148)

摘　　要：目的探究组蛋白去乙酰化酶(HDAC6)对乳鼠心房肌成纤维细胞活化增殖的影响作用。方法将50只乳鼠(雄性)分别提取心房肌成纤维细胞,应用Western blot法测定HDAC6和α-平滑肌肌动蛋白(α-SMA)的表达情况;运用qRT-PCR技术检测HDAC6和α-SMA mRNA的表达情况;MTT法检测心房肌成纤维细胞的细胞增殖活性;使用LipofectamineTM2000 Reagent向心房肌成纤维细胞中转染小干扰RNA HDAC6(siRNA-HDAC6)后观察对心房肌成纤维细胞增殖的影响。结果转染心房肌成纤维细胞siRNA-HDAC6组HDAC6和α-SMA的蛋白表达明显下降;qRT-PCR技术检测siRNA-HDAC6组中HDAC6和α-SMA的mRNA表达明显下降;转染siRNA-HDAC6明显抑制心房肌成纤维细胞的增殖活性。结论靶向沉默HDAC6可抑制心房肌成纤维细胞活化增殖,提示其在心房纤维化中可能发挥调控作用。Objective To explore influence of histone deacetylase 6 （HDAC6） on neonatal rat atrial fibroblasts ac- tivation and proliferation. Methods Atrial fibroblasts were treated with transforming growth factor-β1 （TGF-β1） for 48 hours, qRT-PCR was applied to assess the expression of HDAC6 and a-smooth muscle actin （oL-SMA） mRNA levels. The protein expression levels of HDAC6 and α-SMA were detected by Western blot. MTT assay was used to determine the proliferation of atrial fibroblasts. LipofectamineTM2 000 Reagent was used to transfected atrial fibro- blasts with siRNA-HDAC6. Results After cardiac fibroblasts transfected with siRNA-HDAC6, HDAC6 expression was declined and α-SMA expression was decreased. The atrial fibroblasts treated with TGF-β1 that HDAC6 expres- sion was increased and α-SMA expression was increased. The activity of cardiac fibroblasts was decreased after transfected with siRNA-HDAC6. Conclusion HDAC6 can suppress proliferative activity of atrial fibroblasts markedly and could be a new therapeutic target of preventing atrial remodeling. The application of its regulator will provide the new idea and method for the prevention and cure of atrial fibrosis.

关 键 词：心房肌成纤维细胞 HDAC6 Α-SMA 

分 类 号：R541[医药卫生—心血管疾病]