RP-HPLC-UV法测定山豆根中苦参碱与氧化苦参碱  

Determination of Matrine and Oxymatrine in Sophorae Tonkinensis Radix Et Rhizoma by RP-HPLC-UV

作　　者：张晓燕[1] 孙文博[1] 梁妍[1] 郝小燕[1] 郭治友[2] 周威[1] ZHANG Xiaoyan SUN Wenbo LIANG Yan HAO Xiaoyan GUO Zhiyou ZHOU Wei(School of Basic Medical Science, School of Pharmacy, Guizhou Medical University, Guiyang 550025, Guizhou , China School of Life Science, Qiannan Normal University for Nationalities, Duyun 558000, Guizhou, China)

机构地区：[1]贵州医科大学基础医学院药学院,贵州贵阳550025 [2]黔南民族师范学院生命科学系,贵州都匀558000

出　　处：《贵州医科大学学报》2017年第3期269-274,共6页Journal of Guizhou Medical University

基　　金：国家级大学生创新创业训练项目(201410660004);贵州省大学生创新创业训练项目(201510660024);贵州医科大学博士基金(J-2014-006)

摘　　要：目的:建立一种新的反相高效液相色谱法(RP-HPLC-UV)同时测定苦参碱、氧化苦参碱及其在山豆根中的应用。方法:采用RP-HPLC-UV检测方法,Diamonsil C18(250 mm×4.6 mm,5μm)分析色谱柱,流动相为乙腈(A)-25 mmol/L磷酸二氢钾(KH_2PO_4,B)水溶液(10∶90,V/V),检测波长210 nm,流速1.0 m L/min。结果:苦参碱、氧化苦参碱的浓度与峰面积分别在1.00~120.0 mg/L(R=0.999 7)、1.45~144.0 mg/L(R=0.999 9)浓度范围内线性关系良好,平均加样回收率分别为98.69%、102.18%;山豆根药材中苦参碱平均含量0.81 mg/g(生药量),氧化苦参碱平均含量6.98 mg/g(生药量)。结论:该方法简便、准确、实用,适用于同时分析测定苦参碱、氧化苦参碱及其相关药材。Objective：To establish a new RP-HPLC-UV method for simultaneous determination of matrine and oxymatrine,and to study its application in quality detection of Sophorae Tonkinensis Radix Et Rhizoma. Methods：The RP-HPLC-UV method was chose,the separation was performed on a Diamonsil C18（ 250 × 4. 6 mm,5 μm） analytical column（ 4. 6 mm × 250 mm,5 μm）,and the mobile phase was Acetonitrile-25 mmol/L Potassium dihydrogen phosphate water solution（ 10∶ 90,V/V） at a flow rate of 1. 0 m L/min,detection wavelength of 210 nm. Results：The concentrations and peak areas of matrine and oxymatrine were in concentration ranges of 1. 00 ~ 120. 0 μg/m L（ R = 0. 999 7） and1. 45 ~ 144. 0 μg/m L（ R = 0. 999 9） respectively with good linear relation. Their average sample-added recoveries were 98. 69% and 102. 18% respectively. The average content of matrine in Sophorae Tonkinensis Radix Et Rhizoma was 0. 81 mg/g（ crude drug）,and that of oxymatrine was 6. 98 mg/g（ crude drug）. Conclusion：This established method is simple,accurate,and practical,and is suitable for simultaneous determination of matrine,oxymatrine,and other relavant medicinal materials.

关 键 词：苦参碱 氧化苦参碱 山豆根 液相色谱 

分 类 号：R927[医药卫生—药学]