贵阳市某医院非结核分枝杆菌流行状况分析  

Prevalence Analysis of Nontuberculosis Mycobacterium in a Hospital in Guiyang

作　　者：王颜颜[1,2] 欧维正 夏茂宁 黄劲[4] 秦万 蒙俊 王明栋 明春艳[1] 康颖倩[1] WANG Yanyan OU Weizheng XIA Maoning HUANG Jing QIN Wan MENG Jun WANG Mingdong MING Chunyan KANG Yingqian(Teaching Division of Microbiology, Guizhou Medically University, Guiyang 550025, Guizhou, China Teaching Division of Biochemistry and Molecular Biology, Guizhou Medically University, Guiyang 550025, Guizhou, China Guiyang Public Health Treatment Center , Guiyang 550003, Guizhou, China)

机构地区：[1]贵州医科大学微生物学教研室,贵州贵阳550025 [2]贵州医科大学 [3]贵阳市公共卫生救治中心,贵州贵阳550003 [4]贵州医科大学生化与分子生物学教研室,贵州贵阳550025

出　　处：《贵州医科大学学报》2017年第3期322-326,共5页Journal of Guizhou Medical University

基　　金：国家自然基金地方项目(31260029);贵州省国际科技合作项目(黔科合外G字[2014]7006);贵阳市人民政府-贵州医科大学科学技术联合基金“贵阳市科技局创新团队”项目(GY2015-18);贵州省科技计划项目[黔科合(2010)3154号];贵阳市科技计划项目[筑科合同(2014001)33号]

摘　　要：目的:了解2012-2013年贵阳市非结核分枝杆菌(NTM)的流行情况。方法:对贵阳市某医院2012-2013年就诊的可疑结核病患者进行分枝杆菌的培养鉴定,采用PCR-荧光探针及基因芯片法进行鉴定,并对相关结果进行分析。结果:1 500例患者经分离培养和PCR-荧光探针及基因芯片法鉴定为结核分枝杆菌感染的1 469例,鉴定为NTM感染的31例,占2.06%,其中8株为鸟分枝杆菌,10株为胞内分枝杆菌,9株为龟或脓肿分枝杆菌,偶然分枝杆菌、瘰疬分枝杆菌、堪萨斯分枝杆菌及蟾蜍分枝杆菌各1株,分别占NTM 25.80%、32.25%、29.03%、3.23%、3.23%、3.23%、3.23%;NTM感染的阳性患者中,男女比例为1.21∶1,30~59岁年龄段占61.29%;不同性别的感染高峰年龄段不同,男性以75~89岁、女性则以45~59岁年龄段。结论:贵阳市存在一定比例的NTM菌感染,以鸟分枝杆菌、胞内分枝杆菌、龟/脓肿分枝杆菌为主。Objective：To investigate the prevalence of nontuberculosis mycobacterium（ NTM） in Guiyang city from 2012 to 2013. Methods：NTM culturing and strain identification were adopted in patients with suspected tuberculosis in a hospital of Guiyang during the period from 2012 to 2013,PCR-fluorescent probe and gene chip were adopted for identification and analyzing relevant results.Results：1469 strains were identified as Mycobacterium tuberculosis from 1500 strains of clinical isolated and PCR-fluorescent probe and gene chip method,31 strains were NTM,accounted for 2. 06%.Of which 8 strains were Mycobacterium avium,10 strains were Mycobacterium intracellulare,9 strains were Turtle/Mycobacterium abscessus; while there was only one Mycobacterium fortuitum,Mycobacterium scrofulaceum,Kansas Mycobacterium tuberculosis and Mycobacterium xenopi respectively. The proportion was 25. 80%,32. 25%,29. 03%,3. 23%,3. 23%,3. 23%,3. 23% in NTM. Among the NTM-positive patients,the proportion of male to female was 1. 21：1. Patients at age of 30 ~ 59 was61. 29%. Affection peak age group was different in different genders：male was between 75 to 89 and female was between 45 to 59. Conclusions：There is a certain proportion of NTM affection in Guiyang.Mycobacterium avium,Mycobacterium intracellulare and Turtle/Mycobacterium abscessus were the main pathogenic bacteria.

关 键 词：分枝杆菌 结核 分枝杆菌 鸟 分枝杆菌 非结核 菌种鉴定 基因芯片 

分 类 号：R181.32[医药卫生—流行病学] R181.21[医药卫生—公共卫生与预防医学]