艾拉莫德对胶原诱导性关节炎大鼠模型的抗炎作用  

Anti-inflammatory Effect of Iguratimod on Collagen-induced Arthritis Rat Models

作　　者：黄颖[1] 姚血明[1] 唐芳[1] 徐晖[1] 王莹[1] 马武开[1] HUANG Ying YAO Xue-ming TANG Fang XU Hui WANG Hng MA Wu-kai

机构地区：[1]贵阳中医学院第二附属医院,贵州贵阳550001

出　　处：《风湿病与关节炎》2017年第3期5-7,25,共4页Rheumatism and Arthritis

基　　金：国家自然科学基金资助项目(81460727);贵州省社会公关项目(黔科合SY字〔2013〕3048号);贵州省科技合作计划项目(黔科合LH字〔2015〕7259号)

摘　　要：目的:研究艾拉莫德对牛Ⅱ型胶原诱导性关节炎(CIA)大鼠模型的抗炎作用。方法:将40只6周龄Wistar雌性大鼠随机分成空白对照组、模型对照组、甲氨蝶呤组、艾拉莫德组,每组10只。除空白对照组外,其余各组以Ⅱ型胶原和冰醋酸制备CIA模型。空白对照组和模型对照组给予生理盐水灌胃,甲氨蝶呤组给予甲氨蝶呤2.5 mg·kg^(-1),每周1次灌胃,艾拉莫德组给予艾拉莫德10 mg·kg^(-1)·d^(-1)灌胃。观察各组大鼠关节肿胀度、滑膜病理变化,ELISA法检测艾拉莫德对大鼠血清白细胞介素(IL)-1、IL-1β、肿瘤坏死因子-α(TNF-α)水平的影响。结果:艾拉莫德可明显改善CIA大鼠关节滑膜病理,可明显减轻CIA大鼠关节肿胀,下调血清IL-1、IL-1β、TNF-α水平,且艾拉莫德组优于甲氨蝶呤组,差异有统计学意义(P<0.01或P<0.05)。结论:艾拉莫德对CIA模型鼠关节滑膜有保护及明显的抗炎作用,其机制可能与下调致炎因子IL-1、IL-1β、TNF-α水平有关。Objective：To study the anti-inflammatory effect of Iguratimod on bovine type Ⅱ collageninduced arthritis rat models （ CIA models ） .Methods：Forty six-week female Wistar rats were randomly divided into a blank control group,a model control group,a methotrexate group and an Iguratimod group,with ten rats in each.Except the blank control group,CIA models were established in the other groups with collagen type Ⅱ and glacial acetic acid.The blank control group and the model control group were given intragastric administrations respectively with normal saline,the methotrexate group was given intragastric administrations with 2.5 mg · kg-1 of methotrexate,once a week,while the Iguratimod group was given intragastric administrations with 10 mg · kg-1 · d-1 of Iguratimod, observing the swelling degree and the pathological changes of synovial membrane.ELISA method was used to detect the effects of Iguratimod on the levels of IL-1,IL-1β and TNF-α.Results：Iguratimod could significantly improve the pathology of synovial tissue in CIA rats,obviously reduce their joint swelling,and down regulate the levels of IL-1,IL-1β and TNF-α in them,the Iguratimod group being better than the methotrexate group and the difference being statistically significant （ P 〈 0.01 or P 〈 0.05 ） .Conclusion：Iguratimod has protective and anti-inflammatory effects on CIA rat models,whose mechanism may be related to the down-regulation of inflammatory factors IL- 1 ,IL- 1β and TNF-α.

关 键 词：关节炎 类风湿 胶原诱导性关节炎 艾拉莫德 关节滑膜病理 IL-1 IL-1Β TNF-α 大鼠 

分 类 号：R684.3[医药卫生—骨科学]