边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析  

Study on the Polymorphisms of IGF-ⅠR Gene Alu Ⅰ Locus and Genetic Effect on Growth and Reproduction Traits in Bian Chicken

作　　者：吴鹏飞[1] 王冬[2] 张向前[1] 张丽[3] 丁馥香[3] 张跟喜[1] WU Peng-fei WANG Dong ZHANG Xiang-qian ZHANG Li DIGN Fu-xiang ZHANG Gen-xi(College of Animal Science and Technology , Yangzhou University, Yangzhou 225009, China Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China Shanxi Livestock and Poultry Breeding Station, Taiyuan 030032, China)

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]中国农业科学院家禽研究所,扬州225125 [3]山西省农业科学院畜牧兽医研究所,太原030032

出　　处：《中国畜牧兽医》2017年第3期813-818,共6页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技公关(20140311021-1);国家肉鸡产业技术体(nycytx-42-G1-05);江苏高校优势学科建设工程;扬州大学科技创新培育基金(2016CXJ069)

摘　　要：为了寻找与边鸡生长性状和繁殖性状相关的遗传标记,本试验采用PCR-RFLP技术检测边鸡类胰岛素生长因子Ⅰ受体(insulin-like growth factorⅠreceptor,IGF-ⅠR)基因AluⅠ位点的多态性,并分析该多态位点对边鸡生长性状和繁殖性状的遗传效应。结果显示,边鸡IGF-ⅠR基因AluⅠ位点存在多态性,在外显子2的376bp处有1个G→A突变,产生了GG、GA和AA 3种基因型,基因型频率分别为0.687、0.296和0.017,GG为优势基因型;G等位基因频率为0.835,为优势等位基因。卡方适合性检验结果表明,该基因座位在边鸡群体中处于哈代-温伯格平衡状态(P>0.05)。相关分析结果表明,在8、14、16和18周龄时,GG基因型个体的体重显著高于GA基因型(P<0.05),GG基因型个体的开产体重极显著高于GA基因型个体(P<0.01),但并不能说明该位点可以作为影响边鸡繁殖性状的遗传标记。IGF-ⅠR基因外显子2的AluⅠ位点(G→A)可能是影响边鸡生长性状的一个遗传标记。Single nucleotide polymorphisms（SNPs）in exon 2of insulin-like growth factorⅠ receptor（IGF-ⅠR）gene were detected by PCR-RFLP in Bian chicken,which was to find the correlation markers of growth and reproduction traits in Bian chicken.The results showed that AluⅠsite displayed polymorphic,and a G→A mutation at the position of the 376 bp in exon 2was occurred.There were three genotypes（GG,GA and AA）,the genotypes frequencies were 0.687,0.296 and 0.017,respectively,GG genotype was the dominant genotype,the frequency of G allele was 0.835,which was the dominant allele.This site was in Hardy-Weinberg equilibrium（P〉0.05）.Correlation analysis showed that GG genotype had significantly higher body weight than those of GA genotype at the age of 8,14,16 and 18weeks（P〈0.05）;The GG geno type had extremely significantly higher body weight at first egg than those of GA genotype（P〈0.01）,but it didn＇t mean that this locus could be used as a genetic marker for reproduction traitsin Bian chicken.This results suggested that the AluⅠsite（G→A）in exon 2of IGF-ⅠR gene could be used as a genetic marker for growth traits in Bian chicken.

关 键 词：边鸡 胰岛素样生长因子-Ⅰ受体基因 生长性状 繁殖性状 PCR-RFLP 

分 类 号：S813.3[农业科学—畜牧学]