内蒙古地区羊源大肠杆菌耐药性研究  

Study on the Drug-resistance of Escherichia coli Isolated from Sheep in Inner Mongolia

作　　者：刘正明[1,2] 李金泉[2,3] 黄德浩[1,2] 郝欣蔚 郝普国 吴莉芩 吴烨挺 杨奇妙 赵红霞[1,2] LIU Zheng-ming LI Jin-quan HUANG De-hao HAO Xin-wei HAO Pu-guo WU Li-qin WU Ye-ting YANG Qi-miao ZHAO Hong-xia(College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China The Ministry of Agriculture Laboratory of Clinical Diagnosis and Treatment Technology on Animal Disease, Hohhot 010018, China College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]农业部动物疾病临床诊疗技术重点实验室,呼和浩特010018 [3]内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《中国畜牧兽医》2017年第3期839-846,共8页China Animal Husbandry & Veterinary Medicine

基　　金：博士后基金(110/108989)

摘　　要：为确定内蒙古地区羊源大肠杆菌的耐药表型及其耐药基因的流行情况,本研究采用微量稀释法测定了内蒙古地区108株羊源大肠杆菌对13种临床常用抗菌药物的最小抑菌浓度。结果显示,分离菌株对阿莫西林、头孢噻吩、磺胺甲唑、黏菌素的耐药率最高,均达100.0%,对阿莫西林/克拉维酸、四环素、环丙沙星的耐药率在50%~80%之间,对头孢噻肟、美洛培南、新霉素的耐药率均低于10%,较为敏感。108株羊源大肠杆菌中耐7种以上药物的菌株占94.4%,其中15.6%菌株对13种抗菌药物耐药,只有1株菌对所有抗菌药物敏感。采用PCR方法对羊源大肠杆菌分离株所携带的6种相关耐药基因进行检测,结果显示,6种耐药基因中的4种耐药基因blaTEM、proP-2、sul-Ⅰ、ampG检出率超过50%,耐药基因aph(3′)-Ⅰ携带率达40%,只有耐药基因aac(3)-Ⅱ检出率仅为5.5%。由此可见,内蒙古地区羊源大肠杆菌对13种抗菌药物产生了不同程度的耐药性,且存在严重的多重耐药情况,羊源大肠杆菌分离株携带aph(3′)-Ⅰ、sul-Ⅰ、ampG、blaTEM、proP-2、aac(3)-Ⅱ耐药基因。In order to determine the drug resistance phenotype and prevalence of drug resistance genes in Escherichia coli（E.coli）isolated from sheep in Inner Mongolia,the minimum inhibitory concentrations（MICs）of the isolates to antibiotics commonly used in veterinary clinical were detected by micro-dilution method in vitro.The results showed that the highest resistance rates of the isolates to amoxicillin,cephalothin,sulfamethoxazole and polymyxin were up to 100.0%,respectively.To amoxicillin-clavulanic,tetracycline and ciprofloxacin were 50%to 80%.These isolates were sensitive to cefotaxime,meropenem trihydrate,neomycin,and their resistance rates were all less than 10%.Among the 108 strains of E.coli from sheep,94.4%of them were resistant to more than 7antimicrobial agents,15.6% of them were resistant to 13 antimicrobial agents,only one strain was sensitive to all antimicrobial agents.Six kinds of resistance genes among the108 E.coli isolates were detected by PCR method.The results showed that detection rates of 4kinds of drug resistance genes including blaTEM,proP-2,sul-Ⅰand ampG were all over 50%,the detection rate of resistance gene aph（3′）-Ⅰ was up to 40%,only resistance gene aac（3）-Ⅱdetection rate was 5.5%.Thus,the sheep E.coli isolates in Inner Mongolia produced various degrees of resistance to 13 kinds of antibiotics,and their multi-drug resistances were very serious.They carried aph（3′）-Ⅰ,sul-Ⅰ,ampG,blaTEM,proP-2and aac（3）-Ⅱresistance genes.

关 键 词：羊 大肠杆菌 耐药性 耐药基因 

分 类 号：S826.95[农业科学—畜牧学]