用HPLC法测定不同地区虎杖饮片中的4种有效成分  

Determination of four active ingredients in Polygonum cuspidatumfrom different regions by HPLC method

作　　者：蔺红伟[1] 江春霞[1] 朴淑娟[1] 陈万生[1] LIN HongWei JIANG ChunXia PIAO ShuJuan CHEN WanSheng(Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, Chin)

机构地区：[1]第二军医大学长征医院药学部,上海200003

出　　处：《药学服务与研究》2017年第1期27-30,共4页Pharmaceutical Care and Research

摘　　要：目的:采用HPLC法检测不同地区所产虎杖饮片中的4种有效成分(虎杖苷、白藜芦醇、大黄素和大黄素甲醚)的含量,为鉴别虎杖药材的真伪优劣提供依据。方法:色谱条件为色谱柱Agilent Zorbax SB-C18柱(4.6mm×250mm,5μm);流动相为0.1%甲酸水溶液(A相)-乙腈(B相),梯度洗脱(0~15min,20%B相;15~20min,20%~40%B相;20~30min,40%~70%B相;30~35min,70%~80%B相);检测波长306nm;流速1ml/min;柱温30℃;进样量为10μl。结果:虎杖饮片中的有效成分虎杖苷、白藜芦醇、大黄素和大黄素甲醚分离良好,精密度、重复性和稳定性均符合标准。虎杖苷在9.34~83.74μg/ml、白藜芦醇在4.07~36.65μg/ml、大黄素在3.74~33.62μg/ml、大黄素甲醚在3.62~32.54μg/ml范围内线性关系良好。虎杖苷、白藜芦醇、大黄素和大黄素甲醚的加样回收率分别为(98.21±1.086)%、(97.70±0.802)%、(97.58±1.005)%和(97.38±0.714%)(n=6)。结论:该方法快捷简便、稳定可靠,可用于虎杖饮片中有效成分的含量测定。Objective： To establish a HPLC method for content determination of four active ingredients（polydatin,resveratrol,emodin and physcione） in Polygonura cuspidaturn from different regions, so as to provide reference for quality identification of Polygonum cuspidatum. Methods： An Agilent Zorhax SB-C18 column（4.6 mm×250mm,5μm） was employed with the mobile phase consisting of 0.1% formic acid（phase A）-acetonitrile（phase B）, gradient elution（0-15 min, 20% phase B; 15- 20 min,20%-40% phase B：20-30 min,40%-70% phase B：30-35 min,70%-80% phase B） at a flow rate of 1.0 ml/min. The detection wavelength was 306 nm, the column temperature was 30 ℃, and the sample size was 20 μl. Results： Polydatin, resveratrol, emodin and physcione displayed good linearity （r= 0. 999 9） within the ranges of 9. 34-83.74 μg/ml, 4.07-36.65 μg/ml, 3. 74- 33.62μg/ml and 3.62-32.54μg/ml, respectively. The recovery rates of polydatin, resveratrol, emodin and physcione were respectively （98. 21± 1. 086）%, （97.70 ±0. 802）%, （97. 58±1. 005）% and （97.38 ± 0. 714%）（n= 6）, respectively. Conclusion： The method is rapid, simple, stable and reliable, and could be used for the determination of active constituents in Polygonurn cuspidaturn.

关 键 词：虎杖 虎杖苷 白藜芦醇 大黄素 大黄素甲醚 色谱法 高效液相 

分 类 号：R927.2[医药卫生—药学]