乌拉尔图小麦全基因组NBS-SSR位点分析及标记开发  

Genome-wide analysis and marker development of NBS-SSR in Triticum urartu

作　　者：乔麟轶[1,2] 畅志坚[2] 张晓军[2] 刘静[3] 朱艳[3] 詹海仙[2] 郭慧娟[2] 李欣[2] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西省农业科学院作物科学研究所/作物遗传与分子改良山西省重点实验室,山西太原030031 [3]山西大学生物工程学院,山西太原030006

出　　处：《山西农业大学学报（自然科学版）》2017年第3期153-157,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学青年基金项目(31601303);山西省青年基金项目(2015021145);山西省农业攻关项目(20150311001-1);山西省农科院攻关项目(15YGG01,YGG1602)

摘　　要：[目的]本研究旨在诊断乌拉尔图小麦NBS家族所在基因组序列的SSR位点,并为抗源的发掘和利用开发分子标记。[方法]利用NBS隐马模型文件检索数据库获得目标序列,通过软件诊断序列SSR位点并开发标记,利用乌拉尔图小麦抗白粉病材料UR207和感病材料UR203进行标记验证。[结果]本研究从乌拉尔图小麦全基因组中分离出463个NBS基因,分为N、CN、NL和CNL共4类。从NBS所在scaffold序列上发现4 292个SSR位点,以二碱基重复位点最多,占78.1%。随机开发了42个分布于各染色体臂的TuNBS-SSR标记,有12个标记在UR207和UR203间存在多态性。[结论]本研究从乌拉尔图小麦全基因组中诊断出4 292个NBS-SSR位点,开发的12个标记在抗感材料间有多态性,可用于乌拉尔图小麦材料抗病性鉴定和抗病基因筛选。[Objective]The aim of this study was to diagnose the SSR loci on genome sequences of the NBS family in Triticum urartu and to develop molecular markers for the exploitation and utilization of this resistance resources.[Method]In this paper,the target sequences were getted by searching the database using NBS HMM,and molecular markers were developed based on the SSR loci diagnosed by software,then the markers were verificated by two T.urartu materials which resistant（UR207）and susceptible（UR203）to the Blumeria graminis tritici,respectively.[Results]463NBS genes,which can be divided into four categories containing N,CN,NL and CNL,were separated from the T.urartugenome.4 292 SSR loci were found on the scaffold sequences with NBS and of which the dinucleotide repeat loci is most,accounting for 78.1%.42TuNBS-SSR markers distributing in the each chromosome arm were developed randomly and 12 markers showed polymorphism between UR207 and UR203.[Conclusion]4 292 NBSSSR loci were diagnosed fromT.urartugenome and 12 markers exhibited polymorphism between resistant and susceptible materials.The results can be used for the identification of disease resistance and the screening of resistance genes of T.urartu.

关 键 词：乌拉尔图小麦 NBS类基因 SSR标记 抗病 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]