Inhibitory Effects of S-Nitroso-Glutathione on Sporulation Process of Eimeria tenella Oocysts  

作　　者：Yu Xing-xing Xing Tao Zhang Hong-fei Yao Zheng-xing Zhou Guang-sheng Wang Hai-gen Zhu Ai-jun 

机构地区：[1]Agriculture Service Centre of Pudong District Nanhui New Town,Shanghai 201303,China [2]Minhang Center for Animal Disease Control and Prevention,Shanghai 201109,China [3]Sijia Town Animal Husbandry and Veterinary Station of Haimen City,Haimen 226100,Jiangsu,China [4]Agriculture Service Centre of Pudong District Shuyuan Town,Shanghai 201304,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2017年第1期42-47,共6页东北农业大学学报（英文版）

摘　　要：To study the inhibiting effects of S-Nitroso-Glutathione （GSNO） on sporulation process of Eimeria tenella （E. tenella） oocysts by detecting the sporulation rate, GSNO as nitric oxide donor was used to treat E. tenella oocysts sporulated for different times; Several kinds of antioxidants and oxidants were used to investigate the inhibitory effects of GSNO on sporogony process of E. tenella oocysts. The results showed that GSNO still inhibited the oocysts sporulated 10 h, but had few inhibiting effect on the oocysts sporulated for 14 h; the antioxidants, such as Vitamin C （VC）, mannitol and sodium salicylate, could not eliminate the inhibitory effects of GSNO on oocysts; neither did the ferrous sulfate （FeSO4） nor Dithiothreitol （DTT）. However, the oxidants, potassium dichromate （K2Cr2OT） and potassium permanganate （KMnO4） could obviously repress the effects of GSNO on oocysts. At present, the inhibitory mechanism of GSNO on unsporulated oocysts was still unclear.To study the inhibiting effects of S-Nitroso-Glutathione （GSNO） on sporulation process of Eimeria tenella （E. tenella） oocysts by detecting the sporulation rate, GSNO as nitric oxide donor was used to treat E. tenella oocysts sporulated for different times; Several kinds of antioxidants and oxidants were used to investigate the inhibitory effects of GSNO on sporogony process of E. tenella oocysts. The results showed that GSNO still inhibited the oocysts sporulated 10 h, but had few inhibiting effect on the oocysts sporulated for 14 h; the antioxidants, such as Vitamin C （VC）, mannitol and sodium salicylate, could not eliminate the inhibitory effects of GSNO on oocysts; neither did the ferrous sulfate （FeSO4） nor Dithiothreitol （DTT）. However, the oxidants, potassium dichromate （K2Cr2OT） and potassium permanganate （KMnO4） could obviously repress the effects of GSNO on oocysts. At present, the inhibitory mechanism of GSNO on unsporulated oocysts was still unclear.

关 键 词：S-nitroso-glutathione Eimeria tenella inhibiting characteristic 

分 类 号：S852.729[农业科学—基础兽医学]