维生素D及其受体在吸烟患者中表达水平的研究  

Expression of vitamin D and its receptor in smoking patients

作　　者：丁炎[1] 崔湧[2] 李悦[1] 昌建鈞 葛鑫[1] 聂宏光[1] Ding Yan Cui Yong Li Yue Chang Jianjun Ge Xin Nie Hongguang(Institute of Metabolic Disease Research and Drug Development, China Medical Uni- versity, Shenyang 110122, China)

机构地区：[1]中国医科大学代谢病分子机制与药物研究所,沈阳110122 [2]中国医科大学附属第一医院麻醉科

出　　处：《山西医药杂志》2017年第6期615-617,共3页Shanxi Medical Journal

基　　金：国家自然科学基金(81270098;81670010);辽宁省自然科学基金(2014021093)

摘　　要：目的探讨吸烟患者血清中的维生素D水平及其受体在肺组织中的表达情况,为临床治疗提供参考依据。方法收集临床肺癌手术患者的血清和肺组织,分为吸烟组和对照组。采用酶联免疫吸附试验(ELISA)法测血清中维生素D水平,采用实时定量聚合酶链反应(PCR)和蛋白质印迹法,检测吸烟对维生素D受体的表达水平的影响。结果吸烟组的维生素D水平、维生素D受体的mRNA和蛋白的表达都高于对照组。结论维生素D及其受体水平表达的提高可能参与了慢性阻塞性肺疾病(COPD)患者增强肺脏液体清除的作用,将为以后寻求合适药物减轻COPD患者气道黏液高分泌状态提供理论基础。Objective To explore the expression of vitamin D and its receptor in smoking patients,so as to provide good advice for clinical treatment.Methods Serum samples and lung tissues of patients with lung cancer resection were collected and patients were divided into smoking group and control group.The serum levels of vitamin D were measured by ELISA and the expression levels of VDR mRNA and protein were detected by real-time quantitative PCR and Western blotting,respectively.Results The levels of vitamin D in serum and expression of vitamin D receptor mRNA and protein in smoking group were higher than those in control group.Conclusion The enhancement of vitamin D and its receptor expression may be involved in the alveolar fluid clearance of COPD patients,which may provide a theoretical basis for the clinical treatment of airway mucus hypersecretion in COPD patients.

关 键 词：肺疾病 慢性阻塞性 吸烟 维生素D 

分 类 号：R563.9[医药卫生—呼吸系统]