脂肪组织chemerin及其受体chemerinR基因表达与肥胖及2型糖尿病的关系  

Relationship of adipose chemerin and its receptor chemerinR gene expression to obesity and type 2 diabetes mellitus

作　　者：王玲艳 于浩泳[2] 魏丽[2] WANG Ling-yan YU Hao-yong WEI Li(Department of Endocrinology and Metabolism, Shanghai Fengxian District Central Hospital, Shanghai 201400, China Department of Endocrinology and Metabolism, Shanghai Sixth People＇s Hospital, Shanghai Jiao Tong University, Shanghai Diabetes Institute, Shanghai 200233, China)

机构地区：[1]上海市奉贤区中心医院内分泌代谢科,上海201400 [2]上海交通大学附属第六人民医院内分泌代谢科上海市糖尿病研究所,上海200233

出　　处：《上海交通大学学报（医学版）》2017年第3期352-358,共7页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30670989)~~

摘　　要：目的·探讨中国汉族人群中chemerin及其受体chemerin R与肥胖及2型糖尿病的关系。方法·收集24名腹部择期手术患者作为研究对象,按照体质量指数(BMI)分为正常糖调节正常体重组(NGR-NW)、正常糖调节超重/肥胖组(NGR-OW/OB)和2型糖尿病超重/肥胖组(T2DM-OW/OB)。采用RT-PCR法测定腹部皮下脂肪组织和腹内网膜脂肪组织中chemerin、chemerin R m RNA水平。结果·NGR-OW/OB组和T2DM-OW/OB组腹部皮下脂肪组织和腹内网膜脂肪组织中chemerin m RNA水平均显著高于NGR-NW组(P<0.05)。相关分析显示,网膜脂肪组织chemerin m RNA水平与BMI、空腹胰岛素(FINS)、三酰甘油和血清chemerin浓度呈正相关(r分别为0.577、0.561、0.472、0.623,P均<0.05);腹部皮下脂肪组织中chemerin m RNA水平与BMI、FINS、血清chemerin浓度呈正相关(r分别为0.692、0.513、0.497,P均<0.05)。结论·网膜脂肪组织、皮下脂肪组织的chemerin m RNA的表达量均与BMI、FINS、血清chemerin浓度呈正相关,提示chemerin基因可能在肥胖及2型糖尿病的病理生理机制中发挥一定作用。Objective · To explore the relationship of adipose chemerin and its receptor chemerinR gene expression to obesity and type 2 diabetes mellitus. Methods · Twenty-four patients undergoing elective abdominal surgery were enrolled, and were divided into normal glucose regulation-normal weight group （NGR-NW）, normal glucose regulation-overweight/obesity group （NGR-OW/OB）, and type 2 diabetic overweight/obesity group （T2DMOW/OB） according to the body mass index （BMI）. The levels of chemerin and chemerinR mRNA were detected by reverse transcription PCR （RT-PCR）. Results · Compare to the NGR-NW group, the chemerin mRNA levels of abdominal subcutaneous and omental fat were significantly increased in the NGR-OW/OB and T2DM-OW/OB group （P〈0.05）. Correlation analysis showed that the chemerin mRNA levels of abdominal omental fat were positively correlated with BMI, fasting insulin （FINS）, triglyceride and serum chemerin （r=0.577, r=0.561, r=0.472, r=0.623, P〈0.05 for all）. The chemerin mRNA levels of abdominal subcutaneous fat showed significant positive correlation with BMI, FINS and serum chemerin （r=0.692, r=0.513, r=0.497, P〈0.05 for all）. Conclusion · The chemerin mRNA levels of abdominal subcutaneous and omental fat were positively correlated with BMI, FINS and serum chemerin, suggesting that the chemerin gene may play a crucial role in the pathophysiological mechanism of obesity and type 2 diabetes.

关 键 词：chemerin基因 CHEMERIN R基因 肥胖 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]