艾纳香总黄酮对大鼠皮肤创伤愈合的作用及机制研究  

The Effect of Total Flavonoids Extracted from Blumea balsamifera(L.) DC. on Skin Wound Healing and Its Mechanism of Rats

作　　者：许罗凤 王丹[2,3] 庞玉新 杨全[1] 夏奇峰[2,3,5] 李小婷[1,2,3] 马青松[1,2,3] 

机构地区：[1]广东药科大学,广东广州510006 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [3]海南省艾纳香工程技术研究中心,海南儋州571737 [4]中国中医科学院中药资源中心,北京100700 [5]贵州大学生命科学学院/贵州省中药材重点实验室,贵州贵阳550025

出　　处：《热带农业科学》2017年第1期75-79,83,共6页Chinese Journal of Tropical Agriculture

基　　金：贵州省重大科技专项计划项目(No.黔科合重大专项字[2013]6011号);海南省科技合作专项(No.6011;2013)

摘　　要：探讨艾纳香总黄酮对大鼠皮肤创伤愈合的影响及其作用机制。取大鼠150只,随机分为模型组、京万红组和艾纳香总黄酮高、中、低组,建立大鼠背部双侧圆形创伤模型,连续给药10 d,测定创面面积,计算愈合率;于术后的1、3、5、7、10 d检测创缘组织IL-1、TNF-α表达量并切取创伤边缘皮肤全层,制片,HE染色后镜下观察创面组织形态学的改变。结果表明:艾纳香总黄酮随药物剂量的增加而提高创面愈合百分率,缩短愈合时间,创伤早期增强创面IL-1、TNF-α的表达。艾纳香总黄酮促进大鼠皮肤创伤的愈合机制之一可能是增强创伤早期炎症因子的分泌,从而促进创面的修复愈合。One hundred and fifty Sprague-Dawley rats were selected and divided into five groups： a model control group, a positive control group, three groups of total flavonoids extracted from Blumea balsamifera with different concentrations （high, medium and low） to study the effect of the total flavonoids on their skin wound healing and healing mechanism. The whole layer dorsal skin was removed to establish an acute wound healing model, and the wound rats were treated for consecutive 10 days. The area of wound surface and the rate of wound healing were recorded. The samples of wound healing skin were taken from the rats at 1 d, 3 d, 5 d, 7 d, 10 d after wound. The histopathological change of wound skin was examined under the microscope. The results showed that higher concentrations of the total flavonoids promoted the skin wound healing rate and shortened the healing time. The levels of IL-1 and TNF-α of the total flavonoids groups were significantly increased at the early stage of trauma. The total flavonoids encouraged the wound healing by inflammatory secretion

关 键 词：艾纳香 总黄酮 皮肤创伤 IL-1 TNF-α 

分 类 号：S567.239[农业科学—中草药栽培] R285[农业科学—作物学]