GPSM2过表达对人胰腺癌细胞迁移能力的影响  

Effects of GPSM2 over-expression on migration ability of human pancreatic cancer cells

作　　者：魏彪[1] 张建新[1] 崔磊[1] 瞿建国[1] 钱小宝 党胜春[1] WEI Biao ZHANG Jianxin CUI Lei QU Jian＇guo QIAN Xiaobao DANG Shengchun(Department of General Surgery, the Affiliated Hospital, Jiangsu University, Zhenjiang, Jiangsu 212001, China)

机构地区：[1]江苏大学附属医院普通外科,江苏镇江212001

出　　处：《中国普通外科杂志》2017年第3期311-316,共6页China Journal of General Surgery

基　　金：江苏省自然科学基金资助项目(BK2012704);江苏省博士后科研计划基金资助项目(1302096B)

摘　　要：目的:构建G蛋白信号调节蛋白2(GPSM2)稳定高表达的胰腺癌细胞株,探讨GPSM2与人胰腺癌细胞迁移能力的关系。方法:构建GPSM2基因过表达质粒(pCMV-Tag3B-GPSM2)并鉴定,将人胰腺癌MIA-PaCa-2细胞分别转染pCMV-Tag3B-GPSM2(GPSM2转染组)或p CMV-Tag3B空载体(阴性对照组),以无处理的MIA-Pa Ca-2细胞为空白对照,用RT-PCR检测各组细胞GPSM2 mRNA表达;Westernblot检测各组细胞GPSM2、β-连环蛋白(β-catenin)的表达;用Transwell实验检测各组胰腺癌细胞迁移能力。结果:成功构建了GPSM2稳定高表达的重组细胞株。与空白对照组比较,GPSM2转染组细胞GPSM2 mRNA表达量明显上调,达前者73.3倍、GPSM2、β-catenin蛋白表达量明显升高、迁移细胞计数明显增加(均P<0.05)。此外,胰腺癌细胞中GPSM2与β-catenin的表达水平呈明显的正向线性关系(P<0.05)。阴性对照组与空白对照组间各指标的差异均无统计学意义(均P>0.05)。结论:上调胰腺癌细胞中GPSM2的表达能增加胰腺癌细胞的迁移能力,该作用可能与β-catenin蛋白表达升高有关。Objective： To construct a stable pancreatic cancer cell line with high expression of the G-protein signaling modulator 2 （GPSM2）, for investigating the relationship between GPSM2 and migration ability of human pancreatic cancer cells. Methods： The plasmids over-expressing GPSM2 gene （pCMV-Tag 3B-GPSM2） were constructed and then were identified. Human pancreatic cancer MIA-PaCa-2 cells were transfected with pCMV-Tag 3B-GPSM2 （GPSM2 transfection group） or empW pCMV-Tag 3B vectors （negative control group）, with untreated MIA-PaCa-2 cells as blank control. In each group of cells, the GPSM2 mRNA expressions were measured by RT-PCR, the protein expressions of GPSM2 and β-catenin were determined by Western blot analysis, and the migration abilities were tested by Transwell assay, respectively. Results： The recombinant cell line with stable high GPSM2 expression was successfully constructed. In GPSM2 transfection group compared with blank control group, the GPSM2 mRNA expression was significantly up- regulated, with a 73.3-fold up-regulation, the protein expression levels of GPSM2 and β-catenin were significantly elevated, and the number of migrated cells was significantly increased （all P〈0.05）. In addition, a positive linear relationship existed between GPSM2 and β-catenin expressions in pancreatic cancer cells （P〈0.05）. There was no statistical difference in any of the indexes between negative control group and blank control group （all P〉0.01）. Conclusion： Up-regulating GPSM2 expression in pancreatic cancer cells can increase the migration ability of pancreatic cancer cells, which may be associated with increased β-catenin protein expression.

关 键 词：胰腺肿瘤 GTP酶激活蛋白质类 Β连环素 细胞运动 

分 类 号：R735.9[医药卫生—肿瘤]