碳酸氢钠对嗜水气单胞菌致病性及其主要毒力相关基因表达的影响  

Effect of sodium bicarbonate on the pathogenicity and expression of main related virulence genes in Aeromonas hydrophila

作　　者：陈江凤[1,2,3] 王广军[1] 郁二蒙[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306 [3]贵州大学动物科学学院,贵州贵阳550025

出　　处：《上海海洋大学学报》2017年第2期197-202,共6页Journal of Shanghai Ocean University

基　　金：国家科技支撑计划项目(2012BAD25B01);广东省海洋渔业科技推广专项(B201400C01);中国水产科学研究院基本科研业务费专项(2016HY-ZD0703);国家现代农业产业技术体系建设专项(CARS-46-17)

摘　　要：为探讨碳酸氢钠对嗜水气单胞菌毒力基因表达的影响,利用荧光定量PCR技术,以未添加碳酸氢钠的嗜水气单胞菌为对照组,添加0.024、0.048、0.072、0.096和0.120 mol/L碳酸氢钠的嗜水气单胞菌为实验组,对嗜水气单胞菌的3个主要毒力基因——溶血素基因(AHH-1)、气溶素基因(Aer A)和弹性蛋白酶基因(ahyB)进行相对表达分析。结果表明,5个实验组嗜水气单胞菌的AHH-1、AerA和ahyB表达量均显著低于对照组。且随着碳酸氢钠浓度的增加,基因表达呈逐渐下调趋势。人工感染结果表明,添加碳酸氢钠培养的嗜水气单胞菌对斑马鱼的半致死量明显比对照组高,而且随着碳酸氢钠浓度的增加,嗜水气单胞菌对斑马鱼的半致死量呈现逐渐加大的趋势,表明毒力逐渐减小。To explore the effect of sodium bicarbonate on expression of virulence genes from Aeramonas hydrophila,the relative expression levels of three major virulence genes,including hemolysin gene（ AHH-1）,aerolysin gene（ Aer A）,and elastinase gene（ ahy B） in A. hydrophila growing with sodium bicarbonate at0. 024,0. 048,0. 072,0. 096 and 0. 120 mol / L were analyzed by fluorescent quantification PCR technique and compared with those of A. hydrophila without sodium bicarbonate as control. The result revealed that the expression levels of AHH-1,Aer A and ahy B in A. hydrophila growing with sodium bicarbonate were significantly lower than those of A. hydrophila without sodium bicarbonate. With increasing sodium bicarbonate level,the expression levels of these genes showed a decreasing trend. The results of artificial infection indicated that compared with the A. hydrophila without sodium bicarbonate,median lethal dose of A.hydrophila with sodium bicarbonate was much higher. What ’s more,with increasing sodium bicarbonate concentration,the median lethal dose of A. hydrophila had a trend to increase gradually,and this means that the virulence of A. hydrophila decreased gradually.

关 键 词：嗜水气单胞菌 碳酸氢钠 致病性 FQ-PCR 毒力因子 基因表达 

分 类 号：S917[农业科学—水产科学]