中麻黄UPLC指纹图谱及其不同产区化学成分差异研究  

UPLC Fingerprint and Chemical Components Difference Among Ephedra intermedia from Different Regions

作　　者：马晓辉[1] 卢有媛 张弦飞[2] 黄得栋 朱田田[1] 晋玲[1,3] MA Xiao-hui LU You-yuan ZHANG Xian-fei HUANG De-dong ZHU Tian-tian JIN Ling(Research Institute of Chinese（Tibetan） Medicinal Resources, Gansu University of Chinese Medicine, Lanzhou 730000, China Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicines of Gansu Province, Lanzhou In- stitute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China Key Laboratory of Chemistry and Quality for TCM and Tibetan Medicine in Universities of Gansu Province ,Lanzhou 730000 ,China)

机构地区：[1]甘肃中医药大学中(藏)药资源研究所,甘肃兰州730000 [2]中国科学院兰州化学物理研究所/中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000 [3]甘肃省高校中(藏)药化学质量研究省级重点实验室,甘肃兰州730000

出　　处：《中药材》2016年第10期2217-2220,共4页Journal of Chinese Medicinal Materials

基　　金：2014高校基本科研业务费专项课题(甘财教[2014]63号06课题);甘肃教育厅科研项目(1106B-07);科技基础性工作专项重点项目子课题(SB2007FY020)

摘　　要：目的:建立中麻黄的超高效液相色谱(UPLC)指纹图谱,为其质量控制提供科学依据,同时揭示不同产区化学成分之间的关系。方法:采用UPLC法建立中麻黄指纹图谱,利用内参比峰计算共有峰的相对峰面积。应用SPSS 19.0对不同产地中麻黄的化学成分进行系统聚类。结果:建立了中麻黄药材的UPLC指纹图谱,共标定了14个共有峰,化学成分的系统聚类结果显示,中麻黄化学成分西北产区聚为一类,山西产区聚为一类。结论:建立了稳定、可靠、重复性好的中麻黄指纹图谱,可用于其质量评价;不同产区中麻黄药材化学成分存在一定差异。Objective： To establish the UPLC fingerprint of Ephedra intermedia,and to provide scientific basis for the quality control,meanwhile,to reveal the chemical components relationship of Ephedra intermedia in different regions. Methods： UPLC method was used to establish the fingerprint of Ephedra intermedia,and the relative peak area of common peaks was calculated based on the reference peak. The application of SPSS 19. 0 clustered the chemical components of Ephedra intermedia. Results： The UPLC fingerprint of Ephedra intermedia was established,and 14 common peaks were calibrated. The results of chemical composition systematic cluster analysis showed that the northeast region gathered for a class and Shanxi producing areas gathered for another class. Conclusion： The UPLC fingerprint of Ephedra intermedia is stable,reliable and reproducible,which could be used for quality evaluation.

关 键 词：中麻黄 UPLC 指纹图谱 化学成分 

分 类 号：R284.1[医药卫生—中药学]