满天星异荭草苷对CCl_4致肝纤维化大鼠NF-κB信号通路的影响  

The Effect of Isoorientin-2″-O-α-L-Arabinopyranosyl( IOA) on NF-κB Pathway on CCl_4-Induced Hepatic Fibrosis in Rats

作　　者：陈永欣[1,2] 卢春远 吕淑娟[1] 韦锦斌[1] 林兴[1] 黄权芳[3] CHEN Yong-xin LU Chun-yuan LV Shu-juan WEI Jin-bin LIN Xin HUANG Quan-fang(Pharmceutical College, Guangxi Medical University, Nanning 530021, China Guangxi University of Chinese Medicine, Nanning 530001, China The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530001, China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西中医药大学,广西南宁530001 [3]广西中医药大学第一附属医院,广西南宁530001

出　　处：《中药材》2016年第10期2318-2323,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81260674;81473431);广西自然科学基金(2013GXNSFAA019146)

摘　　要：目的:研究满天星异荭草苷(IOA)对四氯化碳(CCl_4)诱导大鼠肝纤维化的保护作用机制。方法:用CCl_4复制大鼠肝纤维化模型,成模大鼠分为模型组、吡咯烷二硫代氨基甲酸酯(150 mg/kg)阳性对照组及IOA高(2g/kg)、中(1 g/kg)、低(0.5 g/kg)剂量组,另设正常对照组。给药8 w后,血清生化自动检测仪检测各组大鼠血清ALT、AST、ALB、GLB水平;HE染色观察肝脏组织病理学改变;试剂盒检测各组大鼠肝组织TNF-α、IL-1、IL-10水平;免疫组化染色试剂盒检测大鼠肝组织NF-κBp65、TNF-α表达;PCR检测肝组织Col-ⅠmRNA表达。结果:与模型组比较,IOA能显著降低模型大鼠血清ALT、AST水平,提高ALB/GLB(A/G)比值;明显减轻炎症损伤;显著降低肝组织中TNF-α、IL-1含量,提高IL-10含量。此外,IOA能显著抑制肝组织中NF-κBp65、TNF-α蛋白及Col-I mRNA表达。结论:满天星异荭草苷对肝纤维化有保护作用,其机制可能与抑制NF-κB信号通路继而减轻炎症反应有关。Objective： To investigate the protective effect and mechanism of isoorientin-2″-O-α-L-arabinopyranosyl（ IOA） on CCl4-induced liver fibrosis in rats. Methods： The animal model of liver fibrosis was induced by CCl4,and the rats with hepatic fibrosis were randomly divided into model group,pyrrolidine dithiocarbamate（ 150 mg / kg / d） group,high-,medium-and low-dose of IOA（ 2,1 and0. 5 g / kg） groups. Meanwhile,a normal control group was set. After eight weeks of treatment,the levels of serum alanine aminotransferase（ ALT）,aspertate aminotrans（ AST）,albumin（ ALB） and globulin（ GLB） were detected by automatic biochemical analysis. The hepatic pathological changes were observed by HE staining,and the levels of TNF-α,IL-1 and IL-10 were detected by using commercially assay kits. The expression of NF-κBp65 and TNF-α in liver tissues were detected by immunohistochemical analysis. Moreover,the expression of Col-Ⅰ mRNA was detected by Real-time quantitative PCR. Results： Compared to the model group,IOA significantly decreased the levels of serum ALT and AST,and increased the ratio of ALB / GLB. IOA notably attenuated the inflammation injury. Furthermore,IOA significantly decreased the contents of TNF-α and IL-1,while increased the level of IL-10. IOA significantly inhibited the protein expression of NF-κBp65 and TNF-α and the mRNA expression of Col-Ⅰ in liver tissues. Conclusion： IOA can attenuate liver fibrosis by reducing inflammatory response via inhibition of NF-κB pathway.

关 键 词：异荭草苷 四氯化碳 肝纤维化 

分 类 号：R285.5[医药卫生—中药学]