苏木提取物对大鼠移植心脏心肌PDGF-A、VEGF-C含量的影响  

Effect of Sappanwood on Rats' Transplanted Heart and Expression of Growth Factor in The Transplanted Heart

作　　者：杨建飞[1] 周亚滨[2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《成都中医药大学学报》2017年第1期4-8,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家科技重大专项课题(2009ZX09103425);黑龙江省中医药科研项目(ZHY16-002)

摘　　要：目的:通过观察苏木乙酸乙酯提取物对心脏移植模型大鼠心肌病理形态学及生长因子表达的影响,探讨苏木乙酸乙酯提取物对慢性排斥反应的作用机理,为从中药学中研发出新型的免疫抑制剂奠定工作基础。方法:应用改良Ono术式行大鼠腹腔心脏移植,结合CsA灌胃建立心脏移植慢性排斥动物模型。选择移植心脏跳动良好的大鼠随机分为3组。苏木组予苏木乙酸乙酯提取物溶液灌胃;环孢素组予CsA溶液灌胃;模型对照组按体重计算予等体积橄榄油灌胃,连续灌胃7 d后取下移植心脏。观察移植术后受体的一般情况,移植心脏收缩力情况,移植心脏心肌病理形态学改变,并进行慢性排斥反应诊断分级;采用Real-time-PCR法检测各组大鼠移植心脏心肌生长因子PDGF-A、VEGF-C、FGF-1、FGF-2的基因表达水平。结果:1、与模型对照组相比,苏木组和环孢素组PDGF-A的表达水平降低,差异有统计学意义(P<0.05);苏木组和环孢素组移植心脏心肌组织PDGF-A的表达水平相当,差异无统计学意义(P>0.05)。2、与模型对照组相比,苏木组和环孢素组VEGF-C的表达水平降低,差异有统计学意义(P<0.05);苏木组和环孢素组移植心脏心肌组织VEGF-C的表达水平相当,差异无统计学意义(P>0.05)。3、与模型对照组相比,苏木组和环孢素组FGF-1的表达水平降低,差异有统计学意义(P<0.05);与苏木组相比,环孢素组FGF-1的表达水平降低,差异有统计学意义(P<0.05)。4、与模型对照组相比,苏木组和环孢素组FGF-2的表达水平降低,差异有统计学意义(P<0.05);与苏木组相比,环孢素组FGF-2的表达水平降低,差异有统计学意义(P<0.05)。结论:苏木乙酸乙酯提取物能降低大鼠慢性排斥模型移植心肌PDGF-A、VEGF-C的基因表达,作用与CsA相当。苏木乙酸乙酯提取物能降低大鼠慢性排斥模型移植心肌FGF-1、FGF-2的基因表达,作用不及CsA。Objective： By observing the effect of ethyl acetate extracted Chinese medicine sappanwood on the pathomorphology of transplanted heart and the expression of growth factor with chronic rejection after allogenic cardiac transplantation with rats, to preliminarily discess the mechanism of the ethyl acetate extract of sappanwood resist the chronic rejection, laying foundation for the research of new immunosuppressants. Methods： The allogenic cardiac transplantation model was established byusing the improved Ono operation skill and with the CsA. The rats with successful operations were divided into three groups. The rats in sappanwood group were given olive oil containing sappanwood. The rats in experimental group were given olive oil confining CsA. The rats in control group were given olive oil. Observing their general conditions, the survival of the transplanted hearts and the heartbeats. Observing the recipients＇ general conditions and the heartbeats. Observing pathological change of transplanted heart with light microscope, marking according to the degree of chronic rejection. Then detecting the expression levels of PDGF-A, VEGF-C, FGF-1 and FGF-2 in rats＇ myocardial of every group by using the method of Real-time-PCR. Results： 1. Compared with the model control group, PDGF-A was expressed less in Sappanwood group and CsA group, there was a significant difference （P 〈 0. 05） . 2. Compared with the model control group, VEGF-C was expressed less in Sappanwood group and CsA group, there was a significant difference （P 〈 0. 05） . 3. Compared with the model control group, FGF-1 was expressed less in Sappanwood group and CsA group, there was a significant difference （P 〈 0. 05 ） ; Compared with the Sappanwood group, FGF-1 was expressed less in CsA group, there was a significant difference （ P 〈 0. 05 ）. 4. Compared with the model control group, FGF-2 was expressed less in Sappanwood group and CsA group, there was a significant difference （P 〈0. 05 ）; Compared with the Sappanwood g

关 键 词：心脏移植 慢性排斥 苏木乙酸乙酯 生长因子 

分 类 号：R617[医药卫生—外科学] R285[医药卫生—临床医学]