纤维化肾小管上皮细胞miRNA-21表达情况研究  

Stady on the expression of miRNA-21 in renal tubular epithelial cells

作　　者：钟瑜[1] 韩文伦[1] 朱芸芸[1] 徐再春[1] 

机构地区：[1]浙江省立同德医院肾病科

出　　处：《中国生化药物杂志》2017年第3期44-47,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：浙江省自然科学基金(LQ13H050002)

摘　　要：目的 探究miRNA-21与肾纤维化之间的关系。方法 体外培养肾小管上皮细胞（NRK-52E）,采用转化生长因子β1（transforming growth factorβ1,TGF-β1）制作细胞纤维化模型,采用慢病毒转染miRNA-21;加骨形态发生蛋白-7（bone morphogenetic protein-7,BMP-7）干预TGF-β1处理后的NRK-52E细胞,与阴性对照组相比,检测纤维连接蛋白1（fibronectin 1,FN1）,信号转导分子1（SMAD1）,SMAD6,rno-miR-21-5p的基因表达情况。结果 TGF-β1处理后,NRK-52E细胞BMP-7 mRNA及蛋白表达显著降低,FN1mRNA及蛋白表达显著增加（P〈0.05）。BMP-7基因慢病毒载体转染NRK-52E细胞后,BMP-7 mRNA表达显著增加（P〈0.05）,FN1、SMAD6、rno-miRNA-21-5p mRNA明显下降,SMAD1表达增加（P〈0.05）。结论 miRNA21与TGF-β1所诱导的肾小管上皮细胞表型转化（epithelial-to-mesenchymal transition,EMT）进程呈正相关,可能与BMP-7、SMAD1及SMAD6的改变有关。To describe the relationship between miRNA-21 and renal fibrosis.Methods NRK-52E cells cultured in vitro,then constructed fibrosis model induced by transforming growth factorβ1 （ TGF-β1 ） , then bone morphogenetic protein-7 （ BMP-7 ）-containing lentivirus vector was used to transfect the NRK-52E cells to anti-fibrosis, SMAD1, SMAD6, FN1, rno-miR-21-5p gene expression were detected.Results After TGF-β1 treatment, the BMP-7 mRNA and protein expressions were significantly decreased, FN1 mRNA and protein expressions were significantly increased （ P〈0.05 ） . After transfected by BMP-7-containing lentivirus vector, BMP-7 mRNA expression significantly increased（P〈0.05）, FN1, SMAD6 and rno-miRNA-21-5p mRNA decreased, SMAD1 mRNA increased（P〈0.05）.Conclusion Epithelial-to-mesenchymal transition （EMT） is positively correlated with the miRNA21 and TGF-beta 1, its maybe related to the changes of BMP-7, SMAD1 and SMAD6.

关 键 词：肾小管上皮细胞 纤维化 MIRNA-21 TGF-Β1 BMP-7 

分 类 号：R692.6[医药卫生—泌尿科学]