烟草钾离子通道基因NtTPK的克隆及表达分析  

Cloning and expression analysis of potassium channel NtTPK gene in tobacco

作　　者：许力[1] 黄路平[1] 鲁黎明[1] 李立芹[1] 

机构地区：[1]四川农业大学农学院,四川成都625014

出　　处：《浙江农业学报》2017年第3期366-372,共7页Acta Agriculturae Zhejiangensis

基　　金：植物生理学与生物化学国家重点实验室开放课题(SKLPPBKF 1505;SKLPPBKF 1506)

摘　　要：采用同源克隆的方法对烟草品种K326中的一个钾离子通道基因NtTPK进行了研究,该基因序列全长1 317 bp,编码438个氨基酸。实时荧光定量PCR结果表明,该基因在茎中的表达量最高;在低钾处理24 h后该基因表达量最低;200 mmol·L^(-1)NaCl处理24 h后该基因表达量最高;在烟草打顶7 d后,该基因在叶和根中的表达量达到最高。这些结果为进一步研究该基因的功能提供了一定理论基础。A new potassium channel gene NtTPK was cloned from cultivar K326 using homologous cloning strategy.Sequences analysis showed this gene contained 1 317 bp and coded 438 amino acid residues. Quantitative real-time PCR results showed that the highest expression level of this gene was in the stem; and the lowest expression level emerged after 24 h under low potassium treatment; the gene expression reached its peak level after 24 h under 200mmol·L^-1 NaCl treatment; and 7 d after topping,the gene expression showed the highest level in leaves and roots of tobacco. These results provide theoretical bases for further study of the function of potassium channel NtTPK gene.

关 键 词：烟草 钾离子通道 NtTPK 生物信息学 基因表达 

分 类 号：S572[农业科学—烟草工业]