运用计算机软件预测木质部寄生属信号肽  

Prediction of signal peptide of Xylella fastidiosa based on internet software

作　　者：张志超[1] 黄勇 徐可成[1] 宋红[1] 杨俊誉[1] 张彧[1] 黄琼[1,3] 

机构地区：[1]云南农业大学教育部生物多样性与病虫害控制重点实验室,云南昆明650201 [2]云南省医疗器械检验所,云南昆明650034 [3]云南生物资源保护与利用国家重点实验室,云南昆明650201

出　　处：《浙江农业学报》2017年第3期515-520,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(31270067)

摘　　要：利用已经公布的木质部寄生属(Xylella fastidiosa)2 766个基因组数据信息,运用在线计算机预测软件,分析了木质部寄生属(Xylella fastidiosa)的分泌蛋白组。运用Signal P version 2.0预测得到204个开放阅读框架具有编码蛋白质的N-端信号肽;运用TMHMM、GPI-anchored预测具有非跨膜蛋白(或者在N-端信号肽只有1个跨膜区),不具有锚定位点的173个,运用Target P预测除去分泌到线粒体以及位置不确定的分泌蛋白,最终得到X.fastidiosa的分泌蛋白为149个。分泌类信号肽(Sec-type)114个和具双精氨酸结构的信号肽(RR-motif)32个,分别占分泌蛋白的76.5%和21.5%,脂蛋白信号肽3个,仅占2%,没有预测到类前菌毛信号肽和细菌素及外激素信号肽。One approach to rapidly predict the functions of an entire proteome is to utilize genomic database information and prediction software. Thus,we used a set of prediction algorithms to computationally define a potential secretome. In Xylella fastidiosa,the N-terminal signal peptide was predicted by Signal P version 2. 0. Two hundred and four ORFs were predicted to encode proteins with N-terminal signal peptides. Among these,173 were predicted with no transmembrane domain（or a single transmembrane domain at the extreme N-terminus）. Target P was used to eliminate proteins with 19 mitochondrial targeting signals,and 154 was predicted not to be GPI-anchored. The final computationally-predicted Xylella fastidiosa secretome was estimated to consist of up to 149 ORFs. One hundred and fourteen secretary signal peptides and 32 twin-arginine signal peptides which occupied 76. 5% and 21. 5% of secretome respectively,and 3 lipoprotein signal peptides only occupied 2%,preplan-like,bacteriocin and pheromone signal peptides were not found.

关 键 词：木质部寄生属 分泌蛋白 信号肽 

分 类 号：S432.4[农业科学—植物病理学]