HPLC法同时测定人工蛹虫草不同部位中5种核苷类成分的含量  

Simultaneous Determination of 5 Nucleosides from Different Parts of Cultured Cordyceps militaris by HPLC

作　　者：张勇[1] 卓宝松 刘宝岩 康文艺[1] 

机构地区：[1]黄河科技学院纳米研究所,郑州450063 [2]濮阳市天元虫草研究有限公司,河南濮阳457000

出　　处：《中国药房》2017年第9期1254-1257,共4页China Pharmacy

基　　金：河南省产学研项目(No.162107000038);河南省高等学校重点科研项目(No.17B360005)

摘　　要：目的:建立同时测定人工蛹虫草不同部位中5种核苷类成分含量的方法。方法:采用高效液相色谱法。色谱柱为InertSustain C18,流动相为甲醇-0.02 mol/L磷酸二氢钾溶液(梯度洗脱),流速为0.6 m L/min,检测波长为254 nm,柱温为30℃。结果:尿苷、肌苷、鸟苷、腺苷和虫草素检测进样量线性范围分别为0.568~3.408μg(r=0.999 9)、0.284~1.704μg(r=0.999 9)、0.264~1.584μg(r=0.999 9)、0.232~1.392μg(r=0.999 9)、1.672~10.032μg(r=0.999 8);精密度、稳定性、重复性试验的RSD<3.0%;加样回收率分别为98.2%~103.9%(RSD=1.97%,n=9)、96.2%~101.6%(RSD=1.76%,n=9)、96.7%~102.0%(RSD=1.94%,n=9)、95.1%~99.4%(RSD=1.43%,n=9)和95.6%~101.3%(RSD=1.82%,n=9)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于人工蛹虫草中5种核苷类成分含量的同时测定,蛹虫草不同部位中所含核苷类成分含量不同。OBJECTIVE：To establish a method for simultaneous determination of 5 nucleoside from different parts of cultured Cordyceps militaris. METHODS：HPLC method was adopted. The determination was performed on Inert Sustain C18 column with mobile phase consisting of methanol-0.02 mol/L monobasic potassium phosphate solution（gradient elution）at the flow rate of 0.6m L/min. The detection wavelength was set at 254 nm,the column temperature was 30 ℃. RESULTS：The linear ranges of uridine,inosine,guanosine,adenosine and cordycepin were 0.568-3.408 μg（r=0.999 9）,0.284-1.704 μg（r=0.999 9）,0.264-1.584 μg（r=0.999 9）,0.232-1.392 μg（r=0.999 9）and 1.672-10.032 μg（r=0.999 8）,respectively. RSDs of precision,stability and repeatability tests were all lower than 3.0%. The recoveries were 98.2%-103.9%（RSD=1.97%,n=9）,96.2%-101.6%（RSD=1.76%,n=9）,96.7%-102.0%（RSD=1.94%,n=9）,95.1%-99.4%（RSD=1.43%,n=9）and 95.6%-101.3%（RSD=1.82%,n=9）. CONCLUSIONS：The method is simple,precise,stable and repeatable,and can be used for simultaneous determination of 5 nucleoside from cultured C. militaris,the content of nucleosides are different in different parts of C. militaris.

关 键 词：人工蛹虫草 核苷类 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]