马应龙麝香痔疮膏对直肠黏膜急性损伤的修复作用及机制研究  

Study on the Repairing Effects and Mechanism of Musk Hemorrhoids Ointment on Acute Injury of Rectal

作　　者：姜义武 李杰 唐海云 钱珊英 赵冲 

机构地区：[1]东莞常安医院药剂科,广东东莞523560 [2]东莞常安医院护理部,广东东莞523560 [3]东莞常安医院检验科,广东东莞523560 [4]东莞常安医院普外科,广东东莞523560

出　　处：《中国医院用药评价与分析》2017年第2期176-178,共3页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：东莞市科技局项目(No.201610515000147)

摘　　要：目的:探讨马应龙麝香痔疮膏对直肠黏膜急性损伤的修复作用及机制。方法:选取成年雄性SD大鼠240只作为研究对象,按照编号顺序分为对照组与观察组,每组120只。2组大鼠给予全身麻醉,将3%的乙酸棉签经大鼠肛门置入直肠30 s。观察组大鼠在损伤后30 min开始给予马应龙麝香痔疮膏20 mg,1日2次,连用7 d。对照组大鼠无任何药物干预。2组于第7日使用药物后的30 min及24、72、120 h各处死30只大鼠,在15倍放大镜下,观察2组大鼠直肠的形态特征,并采用苏木精-伊红进行染色,观察马应龙麝香痔疮膏对各指标的影响。结果:治疗后24、72、120 h,观察组大鼠直肠黏膜损伤计分显著低于对照组,差异均有统计学意义(P<0.05)。直肠黏膜损伤24 h后,观察组大鼠血管内皮生长因子、基质金属蛋白酶9水平均显著低于对照组;直肠损伤72、120 h,血管内皮生长因子、诱导型一氧化氮合酶、白细胞介素8、基质金属蛋白酶9、缺氧诱导因子-1α和增殖细胞核抗原的阳性表达率均显著低于对照组,差异均有统计学意义(P<0.05)。结论:马应龙麝香痔疮膏能够显著缓解直肠黏膜急性损伤程度,加快损伤愈合速度。OBJECTIVE ：To probe into the repairing effects and mechanism of musk hemorrhoids ointment on acute injury of rectal. METHODS： 240 male SD rats were extracted to be divided into observation group and control group via the serial number, with 120 cases in each. Two groups were general anesthetized with 3% acetic acid cotton swabs for 30 s. The observation group were given 20 mg musk hemorrhoids ointment after injury of 30 min, twice a day for 7 d; while the control group were not treated with any drugs. On the 7th day, after administration of 30 min, 24 h, 72 h and 120 h, 30 rats in each group were killed. Under 15-time magnifier, the morphological characteristics of rectum of two groups were observed. The hematoxylin-eosin stain was used to analyze the effects of musk hemorrhoids ointment on these indexes. RESULTS ： After treatment of 24 h, 72 h and 120 h, the rectal mucosa damage score of observation group was lower than that of control group, with statistically significant difference （P 〈 0. 05）. After rectal mucosa damage of 24 h, the VEGF, MMP9 level of observation group were lower than those of control group ; after rectal mucosa damage of 72 h and 120 h, the positive expression rate of VEGF,iNOS,IL-8 ,MMP9,HIF-ltx,PCNA was significantly lower than that of control group, with statistically significant difference （P 〈 0. 05 ）. CONCLUSIONS： Musk hemorrhoids ointment can significantly relieve the acute rectal mucosal injury and accelerate the speed of wound healing.

关 键 词：直肠黏膜急性损伤 马应龙麝香痔疮膏 修复 作用机制 

分 类 号：R96[医药卫生—药理学]