黄芪总苷对糖尿病小鼠肾脏保护作用的研究  

Protective effect of astragalosides on kidney damage in diabetic mice

作　　者：刘晓云[1] 张文[1] 李卫平[2] 公惠玲[2] 韩佳[3] 栾家杰[1] 

机构地区：[1]皖南医学院第一附属医院药剂科 [2]安徽医科大学基础医学院 [3]徐州医学院附属医院药学部

出　　处：《中国临床药理学与治疗学》2017年第2期132-138,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(81173624);安徽省自然科学基金项目(11040606M201);安徽省国际科技合作项目(12030603007)

摘　　要：目的:研究黄芪总苷(AST)对糖尿病小鼠肾脏的保护作用及其可能的作用机制。方法:链脲佐菌素(STZ)诱导建立糖尿病小鼠模型。随机分为模型组、4-羟基-2,2,6,6-四甲基哌啶组,AST低、中、高三个剂量组。五组动物分别在给药后4周、6周,检测空腹血糖浓度(FBG)及糖化血清蛋白含量(GSP),称量体质量,计算肾脏指数,观察肾脏病理改变,TUNEL法检测肾小球细胞凋亡,RT-PCR法检测肾脏转化生长因子-β_1(TGF-β_1)、IV型胶原蛋白(Col IV)mRNA表达情况。结果:AST能显著降低STZ诱导的糖尿病小鼠FBG、GSP,且呈现时间、剂量依赖性(P<0.01)。给药后6周,各用药组小鼠体质量上升,肾脏指数下降,肾小球PAS阳性分值下降,肾小球细胞凋亡率降低(P<0.01)。与模型组比较,AST中剂量组小鼠肾脏ColⅣ、TGF-β_1mRNA表达水平明显下降(P<0.05)。结论:AST对糖尿病小鼠有肾脏保护作用,其机制可能与其促进肾脏ColⅣ、TGF-β1mRNA表达有关。AIM：To study the effect of astragalosides（AST） on kidney damage and the mechanism in diabetic mice.METHODS：The diabetic mice model was established by intraperitoneal injection with STZ and the model mice were randomly divided into model group,tempol group,AST-L group,AST-M group,and AST-H group.After 4 weeks and 6 weeks of administration,concentration of fasting blood glucose （FBG） and glycosylated serum protein （GSP） were recorded;weight and kidney indexes were calculated;renal pathological changes were observed;glomerular apoptosis and renal TGF-beta 1,Col IⅣ mRNA expressions were detected by TUNEL and RT-PCR method,respectively.RESULTS：AST can significantly reduce the levels of serum FBG and GSP of diabetic mice induced by STZ in time and dose dependent way （P 〈 0.01）.After 6 weeks of administration,the body weight increased,while the kidney index,glomerulus defects and cell apoptosis decreased in all treatment group （P 〈 0.01）.Compared with model group,the high expression of Col Ⅳ mRNA and TGF-β1 mRNA were significantly decreased in kidney of AST 60 mg/kg dose group （P 〈 0.05）.CONCLUSION：There are some protective effects of AST on kidney of experimental diabetic mice,and its mechanism may be related to promote kidney Col Ⅳ,TGF-beta 1 mRNA expression.

关 键 词：糖尿病 黄芪总苷 Ⅳ型胶原蛋白 转化生长因子-Β1 

分 类 号：R965.2[医药卫生—药理学]