液相色谱-串联质谱法测定红花中非法染色物新品红、金胺O和苏丹红Ⅳ含量的研究  

Simultaneous determination of illegal dyes of New fuchsin,Auramine O and Sudan Ⅳ in Carthami Flos by LC-MS/MS

作　　者：李婷婷 刘莉 曾桢 孔卫东 李良[2] 刘芬[3] 刘文剑[2] LI Tingting LIU Li ZENG Zhen KONG Weidong LI Liang LIU Fen LIU Wenjian(Department of Traditional Chi- nese Medieine, Chengdu Institute for Food and Drug Control, Chengdu 610045, China Medical Affairs Office, Hospital of Jiangxi Corps of Chinese Armed Police Force, Nanehang 330006, China College of Pharmacy, Nanehang University, Nanehang 330006, China)

机构地区：[1]成都市食品药品检验研究院中药室,610045 [2]武警江西总队医院医务处,南昌330006 [3]南昌大学药学院,330006

出　　处：《武警医学》2017年第2期178-181,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的建立液相色谱-串联质谱(LC-MS/MS)法同时测定红花中非法染色物新品红、金胺O和苏丹红Ⅳ的含量。方法采用安捷伦EC-C_(18)(2.1 mm×50 mm,1.8μm)色谱柱,流动相为20 mmol/L甲酸铵+0.1%甲酸(A)和乙腈(B),梯度洗脱,流速为0.2 ml/min,柱温为40℃,离子化方式为ESI,检测模式为MRM,选择离子对:(1)新品红m/z 330.3→223.2,m/z330.3→300.2;(2)金胺O m/z 268.2→147.2,m/z 268.2→252.2;(3)苏丹红Ⅳm/z 381.2→224.1,m/z 381.2→91.1。结果该方法新品红、金胺O和苏丹红Ⅳ的线性范围分别为0.1086~271.50 ng/ml、0.1392~348.00 ng/ml和0.1352~338.00 ng/ml,回收率(n=6)分别为97.45%、96.44%和97.58%,RSD分别为0.70%、1.12%和0.89%,精密度、稳定性和重复性的RSD均小于3%。结论该方法提取简单,测定方法定量准确,灵敏度高,适用于红花中非法染色物新品红、金胺O和苏丹红Ⅳ的含量测定。Objective To develop an LC-MS/MS method for simultaneous determination of illegal dyes of New fuchsin,Auramine O and Sudan Ⅳin Carthami Flos. Methods Using Agilent EC-C18（ 2. 1 mm × 50 mm,1. 8 μm） column,the mobile phase consisted of 20 mmol / L ammonium formate ＋ 0. 1% methane acid-acetonitrile using gradient elution. The column temperature was 40 ℃,ionization mode was ESI,and detection mode was MRM. The ion pair selected was：（1）New fuchsin m / z 330. 3→223. 2,m / z 330. 3→300. 2;（2）Auramine O m / z 268. 2→147. 2,m / z 268. 2→252. 2;（3）Sudan Ⅳ m / z 381. 2→224. 1,m / z 381. 2 →91. 1. Results The linear range of New fuchsin,Auramine O and Sudan Ⅳ was 0. 1086-271. 50 ng / ml,0. 1392-348. 00 ng / ml and 0. 1352-338. 00 ng /ml,respectively,the recoveries were 97. 45%,96. 44% and 97. 58%,respectively,and RSD was 0. 70%,1. 12% and 0. 89%,respectively. RSD of precision,stability and repeatability was less than 3%. Conclusions This method of extraction is simple to use,accurate for determination and highly sensitive. This method can be applied to the determination of illegal dyes of New fuchsin,Auramine O and Sudan Ⅳ in Carthami Flos.

关 键 词：LC-MS/MS 红花 新品红 金胺O 苏丹红Ⅳ 

分 类 号：R917[医药卫生—药物分析学]