雷公藤多苷肝毒性血清药物化学的初步研究  

Preliminary Study on Serum Pharmacochemistry of Tripterygium Glycosides Heptotoxicity

作　　者：王晓东[1] 叶桃[2] 金城[2] 肖小河[2] 赵奎君[1] 蒲仕彪 马致洁[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]解放军302医院中西医结合研究中心,北京100039 [3]云南中医学院中药学院,云南昆明650200

出　　处：《中国现代中药》2017年第3期362-371,共10页Modern Chinese Medicine

基　　金：北京市中医管理局项目(QN2014-18);首都中医药研究专项(14ZY12);北京友谊医院院启动项目(YYQDKT2014-20)

摘　　要：目的:通过对雷公藤多苷引起肝毒性入血成分的分析研究,探讨雷公藤潜在的肝毒性物质基础。方法:观察雷公藤多苷临床20倍等效剂量给药大鼠的肝组织病理形态学、血清AST和ALT来评价雷公藤多苷造成肝损伤的客观真实性;采用血清药物化学研究方法,在MSE质谱正负模式下,建立雷公藤多苷UPLC-Q/TOF-MS指纹图谱和其大鼠血清指纹图谱,比较确定雷公藤多苷的入血原型成分。结果:正常组与给药组大鼠肝组织病理切片指标、血清AST和ALT差异有统计学意义,血清指纹图谱的研究共鉴定Othosphenic Acid、Triptotriterpenic Acid、Demethylregelin、Wilforlide、Cangoronine 5个原型成分。结论:初步确定了雷公藤多苷引起肝毒性的入血成分,为其肝毒性物质基础的阐明奠定了基础。Objective： To study the blood components in tripterygium glycosides on liver toxicity and further explore its material basis. Methods： The objective authenticity of liver damage caused by tripterygium glycosides were evaluated by observing the liver histopathology and serum levels of AST, ALT. The fingerprint of Tripterygium glycosides UPLC-Q-TOF/MS was established by serum pharmacochemistry and MSE mode in a mass spectrometer, and the serum ingredients were identified. Results： Compared with the blank group, the rats showed statistically significant differences in liver pathomorphology and serum AST and ALT after administrated Tripterygium extract four weeks. The serum fingerprint identified 5 components include othosphenic acid, triptotriterpenic acid, demethylregelin, wilforlide and cangoronine. Conclusion： The constituents of liver toxicity of tripterygium glycosides absorbed into blood are preliminarily identified, which could lay the foundation for clarification of its materials basis.

关 键 词：雷公藤多苷 肝毒性 血清药物化学 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]