不同规格商品丹参质量比较研究  

Quality Comparation of Various Commercial Products of Salviae Miltiorrhizae Radix et Rhizoma

作　　者：桑梦如 黄楚璇 吴啟南[1] 樊修和 乐巍[1] 吴达维 许一鸣[1] 

机构地区：[1]南京中医药大学药学院,江苏省中药资源产业化过程协同创新中心,中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023

出　　处：《中国现代中药》2017年第3期430-435,共6页Modern Chinese Medicine

基　　金：江苏省高校优势学科建设工程资助项目(2014);国家公益性行业专项(201407002);国家公共卫生行业专项(2014002)

摘　　要：目的:建立同时测定商品丹参中丹参素、原儿茶酸、原儿茶醛、丹酚酸B、丹酚酸A、二氢丹参酮Ⅰ、丹参酮Ⅰ、隐丹参酮、丹参酮ⅡA含量的高效液相色谱方法,并对20批不同产地的商品丹参进行质量评价。方法:收集自浙江、山东、江苏、河北、河南、四川等不同产区栽培及野生丹参20批。采用Waters x BridgeTMC_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇(A)-0.03%磷酸水溶液(B),梯度洗脱;柱温25℃;检测波长280 nm。通过DPS统计软件进行聚类分析,对20批丹参有效成分含量进行综合评价。结果:上述9种有效成分在一定范围内具有较好的线性关系。精密度、重复性、稳定性的RSD值均小于3%,加样回收率为95.13%~101.35%。从产地看,河南、山东产丹参质量最优,四川丹参品质亦佳,河北、江苏、浙江产者次之。直接购自丹参药材产地者,有效成分含量高于购自药房者。结论:不同规格商品丹参9种有效成分的含量差异较大,其中山东、河南丹参中丹参酮类和丹酚酸成分含量较高。HPLC法同时测定丹参中9种有效成分含量,可更加全面地评价商品丹参的质量。Objective： The study is aimed to establish an HPLC method for simultaneous determination of tanshinol, protocatechuic acid, protocatechualdehyde, salvianolic acid B, salvianolic acid A, dihydrotanshinone Ⅰ, tanshinone Ⅰ, cryptotanshinone and tanshinone ⅡA in commercial products of Salviae Miltiorrhizae Radix et Rhizoma （CPSMR） , and evaluate the qualities of 20 samples from different specifications of CPSMR. Methods ： 20 batches of S. miltiorrhiza were collected from Zhejiang, Shandong, Jiangsu, Hebei, Henan, Sichuan and other habitats. The determination was performed on a Waters xBridgeTM C18 column（250 mm× 4. 6 mm, 5μm）with a gradient mobile phase consisting of methanol（A）-0. 03% phosphoric acid solution（B） at a flow rate of 1.0 mL·min^-1. The column temperature was 25℃ and the detection wavelength was 270 nm. Cluster analysis was conducted with DPS software for comprehensive quality evaluation of 20 batches of CPSMR. Results ： All the 9 effective constituents showed good linearity. The RSD of the precision, repeatability and stability tests were less than 3%. The average recoveries were in the range of 95. 13% -101.35%. In view of habitats, CPSMR from Sichuan province were better than those from Hebei, Jiangsu and Zhejiang province. CPSMR from Henan and Shandong have the samples purchased from pharmacies. Conclusion ： The contents of 9 effective constituents are different among Salviae Mihiorrhizae Radix et Rhizoma of differet specifications in markets from various habitats. SMRR products from Shandong and Henan have higher contents of tanshinones and salvianolic acids. HPLC can be used for the simultaneous determination of 9 effective constituents in SMRR products, which could be applied for the comprehensive quality evaluation of SMRR products.

关 键 词：丹参 高效液相色谱法 多成分 含量测定 质量评价 

分 类 号：R286.0[医药卫生—中药学]