基于WS_2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究  

Direct Electrochemistry of Glucose Oxidase Based on WS_2 Quantum Dots and Its Biosensing Application

作　　者：李晨露[1] 彭花萍[2] 黄种南 盛依伦[2] 吴佩文[3] 林新华[2] LI Chen-lu PENG Hua-ping HUANG Zhong-nan SHENG Yi-lun WU Pei-wen LIN Xin-hua(Department of High Pressure Oxygen, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005, China Department of Pharmaceutical Analysis of the Fujian Medical University, Fuzhou 350122,China Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China)

机构地区：[1]福建医科大学附属第一医院高压氧室,福建福州350005 [2]福建医科大学药学院药物分析系,福建福州350122 [3]福建医科大学附属第一医院内分泌科,福建福州350005

出　　处：《电化学》2017年第1期53-58,共6页Journal of Electrochemistry

基　　金：国家自然科学基金项目(No.21405015;No.81400805);福建省自然科学基金资助项目(No.2014J05092;No.2016J01449);大学生创新创业训练项目基金(No.C1634)资助

摘　　要：采用水热法制备水溶性WS_2量子点(WS_2QDs)材料,并将该材料进一步用于葡萄糖氧化酶(GOx)的有效固定,构建GOx/WS_2QDs/GCE传感界面.采用透射电镜、紫外-可见光谱和电化学等方法对材料的形貌、GOx的固定化过程,以及传感器的直接电化学和电催化性能进行了表征.结果表明,WS_2QDs材料能够有效促进GOx与电极之间的直接电子转移.基于该传感器对葡萄糖良好的电催化作用,该方法有效实现了对葡萄糖的高灵敏检测,其线性范围为25~100μmol·L^(-1)和100~600μmol·L^(-1),检测限为5.0μmol·L^(-1)(S/N=3).该传感器具有良好的选择性、重现性和稳定性,可用于实际样品血糖的分析测定.In this study,a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase（GOx） on t ungsten disulfide quantum dots（WS2QDs） on the surface of glassy carbon electrode（GCE）.Transmission electron microscopy,UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology,structure,and electrochemical behaviors of the as-prepared WS2QDs and the biofilm modified electrode.The results suggested that the WS2QDs could accelerate the electron transfer between the electrode and the immobilized enzyme,which enabled the direct electrochemistry of GOx without any electron mediator.Besides,the immobilized GOx in WS2QDs film exhibited excellent electrocatalytic activity toward oxidation of glucose due to the excellent biocompatibility of the WS2QDs.The proposed GOx/WS2QDs biofilm electrode exhibited a linear response to glucose concentration in the ranges of 25 ~ 100 μmol·L-1 and 100 ~ 600 μmol·L-1,and the detection limit of the biosensor was as low as 5.0 μmol·L-1.The biosensor also exhibited good selectivity,reproducibility and long-term stability,which might be potenially used for the detection of the real samples.

关 键 词：WS2量子点 葡萄糖氧化酶 直接电化学 生物传感器 

分 类 号：O657.1[理学—分析化学]