恒山黄芪内生真菌Penicillium sp.中gliotoxin类代谢产物的分离和生物活性  

Gliotoxins Isolated from Endophyic Penicillium sp. of Astragali Radix and Their Antimicrobial Activity

作　　者：张弘弛[1] 刘瑞[1] 李慧[1] 安志鹏[1] 周凤[1] 

机构地区：[1]大同大学生命科学学院,应用生物技术研究所,山西大同037009

出　　处：《中国实验方剂学杂志》2017年第7期68-72,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省科技攻关项目(20130311011-6);大同市科技攻关项目(201468-1)

摘　　要：目的:研究恒山黄芪内生真菌Penicillium sp.的gliotoxin类代谢产物和其对黄芪白粉病原菌Erysiphe pisi.的抑制作用。方法:次生代谢产物的分离纯化采用反复硅胶柱色谱法,Sephadex LH-20凝胶色谱法和HPLC方法,并通过化学方法结合核磁共振分析和质谱鉴定其化学结构,抑菌活性测试方法采用最小抑制浓度法。结果:从恒山黄芪内生真菌Penicillium sp.的固体发酵萃取物中分离得到7个gliotoxin类代谢产物,经波谱分析分别鉴定为gliotoxin(1),bisdethiobis(methylthio)gliotoxin(2),dehydroxybisdethiobis(methylthio)gliotoxin(3),dehydrogliotoxin(4),6-deoxy-5a,6-didehydrogliotoxin(5),didehydrobisdethiobis(methylthio)gliotoxin(6),bis-N-norgliovietin(7),抑菌活性测试结果显示,化合物1,4和5对黄芪白粉病原菌显示出强抑制作用,最小抑菌浓度(MIC)分别达到1.56,0.78,6.25 mg·L-1。结论:所有化合物都是首次从黄芪内生真菌发酵产物中分离得到,黄芪内生真菌Penicillium sp.可以作为潜在的产生gliotoxin类代谢产物的菌种资源。Objective： To study on gliotoxin metabolites from endophytic Penicillium sp. of Astragali Radix and investigate their antimicrobial activity to Erysiphe pisi. Method： The compounds were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel chromatography, and HPLC method; their chemical structures were identified by chemical methods combined with nuclear magnetic resonance analysis and mass spectrometry. The MIC values for E. pisi. were determined as sensitivity to the compounds in biological evaluation. Result： Seven gliotoxins were obtained and elucidated as gliotoxin （1）, bisdethiobis （methylthio） gliotoxin （ 2 ）, dehydroxybisdethiobis （ methylthio ） gliotoxin （ 3 ）, dehydrogliotoxin （ 4 ）, 6-deoxy-5a, 6- didehydrogliotoxin （ 5 ） , didehydrobisdethiobis （ methyhhio ） gliotoxin （ 6 ） , and bis-N-norgliovietin （ 7 ）. Bacteriostasic activity test results showed that compounds 1, 4 and 5 had strong inhibitory activity for E. pisi, MIC values of 1.56, 0.78 and 6.25 mg·L^-1. Conclusion： All compounds were isolated from the endophytic fungi of Astragali Radix for the first time, and the endophytic fungus Aspergillus sp. from the stems of Astragali Radix, is a promising source for gfiotoxins.

关 键 词：内生真菌 黄芪 胶霉毒素 黄芪白粉病 

分 类 号：R284.1[医药卫生—中药学]